Identification of Th2-type suppressor T cells among in vivo expanded ocular T cells in mice with experimental autoimmune uveoretinitis.

Abstract

Experimental autoimmune uveoretinitis (EAU), which is a T cell mediated organ specific autoimmune disease, is induced by immunization with interphotoreceptor retinoid binding protein (IRBP) in susceptible strains of mice. It has been found that IRBP-derived peptide 518-529 (p518-529) generates Th2-type responses and inhibits IRBP-induced EAU, indicating that the p518-529 might be an epitope for suppressor T cells in IRBP-induced EAU. First, we observed that there were T cells producing the Th2 type cytokines such as IL-4 and IL-10 in late phase of EAU. Furthermore, to examine whether p518-529-reactive T cells expand in the eye during EAU, T cell receptor (TCR) of ocular T cells was compared with that of p518-529 reactive T cells in spleen from mice with EAU by PCR-single strand conformation polymorphism (PCR-SSCP) and nucleotide sequence analysis. SSCP and sequence analyses indicated that p518-529 reactive TCR BV10+ T cells bearing amino acid motif(PWG) and TCR BV13+ T cells bearing amino acid motif(PGLGGY) in their complementary-determining region 3 (CDR3) region were clonally expanding in ocular tissues on day 28 after immunization, although these T cells were not detected on day 14. These findings demonstrate that p518-529 reactive Th2-type T cells expand oligoclonally in the uveitic eyes in the late stage of EAU and may function as Th2-type suppressor T cells for improvement of the disease.

• Authors: H Keino, M Takeuchi, J Suzuki, S Kojo, J Sakai, K Nishioka, T Sumida, M Usui
• Journal: Clinical and experimental immunology (Clin Exp Immunol) Vol. 124 Issue 1 Pg. 1-8 (Apr 2001) ISSN: 0009-9104 [Print] England
• PMID: 11359436 (Publication Type: Journal Article)

Chemical References

• Autoantigens
• DNA, Complementary
• Epitopes
• Eye Proteins
• Peptide Fragments
• RNA, Messenger
• Receptors, Antigen, T-Cell
• Retinol-Binding Proteins
• interphotoreceptor retinoid binding protein (518-529)
• interstitial retinol-binding protein
• Interleukin-10
• Interleukin-4
• Interferon-gamma

Topics

• Amino Acid Motifs
• Animals
• Autoantigens (immunology)
• Clone Cells (immunology, metabolism)
• DNA, Complementary (genetics)
• Epitopes (immunology)
• Eye Proteins
• Female
• Immunization
• Interferon-gamma (biosynthesis, genetics)
• Interleukin-10 (biosynthesis, genetics)
• Interleukin-4 (biosynthesis, genetics)
• Mice
• Nervous System Autoimmune Disease, Experimental (immunology, pathology)
• Peptide Fragments (immunology)
• Polymerase Chain Reaction
• Polymorphism, Single-Stranded Conformational
• RNA, Messenger (analysis)
• Receptors, Antigen, T-Cell (chemistry, immunology)
• Retina (immunology, pathology)
• Retinitis (immunology, pathology)
• Retinol-Binding Proteins (immunology)
• Specific Pathogen-Free Organisms
• Spleen (immunology, pathology)
• T-Lymphocytes, Regulatory (metabolism, pathology)
• Th2 Cells (metabolism, pathology)
• Uvea (immunology, pathology)
• Uveitis (immunology, pathology)