Dengue virus infects human endothelial cells and induces IL-6 and IL-8 production.

Abstract

In this study dengue virus (DV) was found to infect primary endothelial cells derived from human umbilical cord veins (HUVEC) and alter their cytokine production. Dengue virus infection of HUVEC was confirmed by an increase in plaque-forming units in the culture supernatant and by immunofluorescence assay. HUVEC produced large amounts of interleukin (IL)-6 and IL-8 but not IL-1beta after DV infection. Both the replication of DV and the production of IL-6 and IL-8 by HUVEC after DV infection were inhibited by ribavirin, an antiviral synthetic guanosine analogue. Additionally, increased serum levels of IL-6 and IL-8 were observed in patients with dengue hemorrhagic fever but not dengue fever. Therefore, our results suggest that endothelial cells can be a target for DV infection, and that DV-induced IL-6 and IL-8 production by endothelial cells may contribute to the pathogenesis of dengue hemorrhagic fever.

• Authors: Y H Huang, H Y Lei, H S Liu, Y S Lin, C C Liu, T M Yeh
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) 2000 Jul-Aug Vol. 63 Issue 1-2 Pg. 71-5 ISSN: 0002-9637 [Print] United States
• PMID: 11357999 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• Interleukin-6
• Interleukin-8
• Ribavirin

Topics

• Adolescent
• Adult
• Aged
• Antiviral Agents (pharmacology)
• Child
• Child, Preschool
• Dengue Virus (drug effects, pathogenicity)
• Endothelium, Vascular (drug effects, immunology, virology)
• Female
• Fluorescent Antibody Technique
• Humans
• Interleukin-6 (biosynthesis, blood)
• Interleukin-8 (biosynthesis, blood)
• Male
• Middle Aged
• Ribavirin (pharmacology)
• Severe Dengue (blood, virology)