Physical and radiation hybrid mapping of canine chromosome 12, in a region corresponding to human chromosome 6p12-q12.

Abstract	The positional cloning of the hypocretin receptor 2, the gene for autosomal recessive canine narcolepsy, has led to the development of a physical map spanning a large portion of canine chromosome 12 (CFA12), in a region corresponding to human chromosome 6p12-q13. More than 40 expressed sequence tags (ESTs) were used in homology search experiments, together with chromosome walking, to build both physical and radiation hybrid maps of the CFA12 13-21 region. The resulting map of bacterial artificial chromosome ends, ESTs, and microsatellite markers represents the longest continuous high-density map of the dog genome reported to date. These data further establish the dog as a system for studying disease genes of interest to human populations and highlight feasible approaches for positional cloning of disease genes in organisms where genomic resources are limited.
Authors	R Li, J H Faraco, L Lin, X Lin, L Hinton, W Rogers, J K Lowe, E A Ostrander, E Mignot
Journal	Genomics (Genomics) Vol. 73 Issue 3 Pg. 299-315 (May 01 2001) ISSN: 0888-7543 [Print] United States
PMID	11350122 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Copyright	Copyright 2001 Academic Press.
Chemical References	Autoantigens Carrier Proteins Cytoskeletal Proteins DNA Primers DST protein, human Dystonin Nerve Tissue Proteins Non-Fibrillar Collagens Orexin Receptors Receptors, G-Protein-Coupled Receptors, Neuropeptide collagen type XVII Collagen
Topics	Animals Autoantigens (genetics) Carrier Proteins Chromosomes, Artificial, Bacterial (genetics) Chromosomes, Human, Pair 6 (genetics) Collagen (genetics) Contig Mapping Cytoskeletal Proteins DNA Primers Dogs Dystonin Expressed Sequence Tags Genetic Linkage (genetics) Humans Microsatellite Repeats (genetics) Narcolepsy (genetics) Nerve Tissue Proteins Non-Fibrillar Collagens Orexin Receptors Polymorphism, Genetic (genetics) Radiation Hybrid Mapping Receptors, G-Protein-Coupled Receptors, Neuropeptide (genetics) Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sequence Tagged Sites

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