Impact of Celsior solution on hepatocellular, reperfusion and endothelial cell injury after liver transplantation.

Abstract

BACKGROUND:

Initially, preservation solutions were developed to maintain cell function of the transplanted organs. However, recently developed preservation solutions also contain a variety of substances to reduce the reperfusion injury.

AIM:

To study the effect of three different preservation solutions on the liver cell injury, endothelial cell function and reperfusion injury after liver transplantation.

MATERIAL AND METHODS:

Large White X Landrace pigs of either sex were subjected to orthotopic liver transplantation. Donor livers were flushed and stored in University of Wisconsin Solution, Eurocollins Solution or Celsior Solution for 3 hours. Blood samples were taken at various times post transplantation for assessment of aspartate aminotransferase, hyaluronic acid, malondialdehyde and Vitamin A levels.

RESULTS:

Serum aspartate aminotransferase levels were lower in the livers preserved in the Wisconsin solution. Plasma malondialdehyde levels were lower and serum Vitamin A levels were higher in the livers preserved in Celsior solution. Serum hyaluronic acid levels increased after liver transplantation but were similar with all three solutions.

CONCLUSIONS:

There was less hepato-cellular injury in the livers preserved in Wisconsin solution and less reperfusion injury with the Celsior solution. The endothelial cell injury was similar with all three solutions.

• Authors: A B van As, Z Lotz, M Tyler, D Kahn
• Journal: Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver (Dig Liver Dis) Vol. 33 Issue 2 Pg. 181-6 (Mar 2001) ISSN: 1590-8658 [Print] Netherlands
• PMID: 11346148 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Celsior
• Disaccharides
• Electrolytes
• Glutamates
• Organ Preservation Solutions
• Vitamin A
• Mannitol
• Histidine
• Malondialdehyde
• Hyaluronic Acid
• Aspartate Aminotransferases
• Glutathione

Topics

• Animals
• Aspartate Aminotransferases (analysis)
• Disaccharides (pharmacology)
• Disease Models, Animal
• Electrolytes (pharmacology)
• Endothelium (cytology, drug effects)
• Female
• Glutamates (pharmacology)
• Glutathione (pharmacology)
• Graft Rejection
• Graft Survival
• Histidine (pharmacology)
• Hyaluronic Acid (analysis)
• Liver Transplantation (methods)
• Male
• Malondialdehyde (analysis)
• Mannitol (pharmacology)
• Organ Preservation
• Organ Preservation Solutions (chemistry, pharmacology)
• Probability
• Reperfusion Injury (prevention & control)
• Sensitivity and Specificity
• Swine
• Transplantation, Homologous
• Vitamin A (analysis)