Abstract | PURPOSE: MATERIALS AND METHODS: RESULTS:
TGF-beta bioassay showed that obstructed kidneys in controls contained significantly higher mean TGF-beta plus or minus standard deviation than unobstructed kidneys in controls (73.7 +/- 13.6 versus 14.1 +/- 5.5 pg./mg. tissue) and tranilast significantly decreased tissue TGF-beta in obstructed kidneys (15.9 +/- 4.8 pg./mg. tissue). The terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling assay demonstrated that obstructed kidneys in controls had significantly more mean tubular apoptosis than the unobstructed counterparts (36.6 +/- 6.7 versus 5.8 +/- 5.5 nuclei per high power field) and tranilast significantly decreased mean renal tubular apoptosis in obstructed kidneys (16.2 +/- 1.7 nuclei per high power field). In addition, immunostaining proliferating cell nuclear antigen showed that obstructed kidneys in controls had significantly more mean renal tubular proliferation than unobstructed kidneys (20.7 +/- 3.4 versus 6.2 +/- 2.1 per high power field) and tranilast significantly increased proliferating renal tubules in obstructed and unobstructed kidneys (26.5 +/- 8.3 and 14.5 +/- 3.4 per high power field, respectively). Control obstructed kidneys exhibited significantly more fibrosis, which was also blunted by tranilast. CONCLUSIONS:
Tranilast significantly decreases tissue TGF-beta, resulting in a reduction in tubular apoptosis and an increase in tubular proliferation. This finding suggests that tranilast is a promising agent for preventing renal tubular damage in unilateral ureteral obstruction.
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Authors | A Miyajima, T Asano, T Asano, I Yoshimura, K Seta, M Hayakawa |
Journal | The Journal of urology
(J Urol)
Vol. 165
Issue 5
Pg. 1714-8
(May 2001)
ISSN: 0022-5347 [Print] United States |
PMID | 11342962
(Publication Type: Journal Article)
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Chemical References |
- Anti-Allergic Agents
- Proliferating Cell Nuclear Antigen
- Transforming Growth Factor beta
- ortho-Aminobenzoates
- Collagen
- tranilast
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Topics |
- Animals
- Anti-Allergic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Biological Assay
- Cell Division
(drug effects)
- Cell Line
- Collagen
(analysis)
- Fibrosis
- Immunohistochemistry
- In Situ Nick-End Labeling
- Kidney
(metabolism)
- Kidney Tubules
(pathology)
- Lung
- Mink
- Proliferating Cell Nuclear Antigen
(analysis)
- Rats
- Rats, Sprague-Dawley
- Transforming Growth Factor beta
(analysis, antagonists & inhibitors)
- Ureteral Obstruction
(pathology)
- ortho-Aminobenzoates
(pharmacology)
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