Identification of human and mouse HIRA-interacting protein-5 (HIRIP5), two mammalian representatives in a family of phylogenetically conserved proteins with a role in the biogenesis of Fe/S proteins.

Abstract	The human HIRA protein is encoded from a region of chromosome 22q that is critical for the DiGeorge syndrome and the velocardiofacial syndrome. We have previously reported that it directly interacts with core histones, with a novel histone-binding protein, HIRIP3, and during mouse embryogenesis, with the developmentally regulated homeodomain protein Pax3, suggesting a promoter-targeted function at the chromatin level. We here report on HIRA-interacting protein 5 (HIRIP5), a small acidic protein that interacted with HIRA in a double-hybrid screen performed in yeast and in in vitro protein interaction experiments. HIRIP5 has highly conserved homologs in both prokaryotes and eukaryotes, including the NFU1 gene product which has been implicated in iron metabolism in mitochondria of the yeast Saccharomyces cerevisiae. By radioactive in situ hybridization, the HIRIP5 gene was mapped to the 2p13-p15 chromosomal region, separate from any region previously associated with DiGeorge syndrome.
Authors	S Lorain, Y Lécluse, C Scamps, M G Mattéi, M Lipinski
Journal	Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1517 Issue 3 Pg. 376-83 (Feb 16 2001) ISSN: 0006-3002 [Print] Netherlands
PMID	11342215 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Carrier Proteins Cell Cycle Proteins DNA, Complementary HIRA protein, human Hira protein, mouse Hirip5 protein, mouse Histone Chaperones Iron-Sulfur Proteins NFU1 protein, human Nuclear Proteins Transcription Factors
Topics	Amino Acid Sequence Animals Base Sequence Carrier Proteins (genetics, metabolism) Cell Cycle Proteins Chromosome Mapping Conserved Sequence DNA, Complementary (genetics) HeLa Cells Histone Chaperones Humans In Situ Hybridization In Vitro Techniques Iron-Sulfur Proteins (biosynthesis) Mice Molecular Sequence Data Nuclear Proteins (genetics, metabolism) Phylogeny Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Transcription Factors (genetics, metabolism) Two-Hybrid System Techniques

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