Laser-induced noninvasive vascular injury models in mice generate platelet- and coagulation-dependent thrombi.

Abstract	A minimally invasive laser-induced injury model is described to study thrombus development in mice in vivo. The protocol involves focusing the beam of an argon-ion laser through a compound microscope on the vasculature of a mouse ear that is sufficiently thin such that blood flow can be visualized by intravital microscopy. Two distinct injury models have been established. The first involves direct laser illumination with a short, high-intensity pulse. In this case, thrombus formation is inhibited by the GPIIb/IIIa antagonist, G4120. However, the anticoagulants, hirulog, PPACK, and NapC2 have minimal effect. This indicates that thrombus development induced by this model mainly involves platelet interactions. The second model involves low-intensity laser illumination of mice injected with Rose Bengal dye to induce photochemical injury in the region of laser illumination. Thrombi generated by this latter procedure have a slower development and are inhibited by both anticoagulant and anti-platelet compounds.
Authors	E D Rosen, S Raymond, A Zollman, F Noria, M Sandoval-Cooper, A Shulman, J L Merz, F J Castellino
Journal	The American journal of pathology (Am J Pathol) Vol. 158 Issue 5 Pg. 1613-22 (May 2001) ISSN: 0002-9440 [Print] United States
PMID	11337359 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Amino Acid Chloromethyl Ketones Antithrombins Fibrinolytic Agents Fluorescent Dyes Peptides, Cyclic Platelet Aggregation Inhibitors Sulfoxides G 4120 Rose Bengal phenylalanyl-prolyl-arginine-chloromethyl ketone
Topics	Amino Acid Chloromethyl Ketones (pharmacology) Animals Antithrombins (pharmacology) Blood Coagulation (drug effects, physiology) Blood Platelets (drug effects, physiology) Blood Vessels (drug effects, pathology, ultrastructure) Disease Models, Animal Fibrinolytic Agents (pharmacology) Fluorescent Dyes (pharmacology) Lasers (adverse effects) Mice Mice, Inbred C57BL Microscopy, Electron Peptides, Cyclic (pharmacology) Platelet Aggregation Inhibitors (pharmacology) Rose Bengal (pharmacology) Sulfoxides (pharmacology) Thrombosis (etiology, pathology, prevention & control) Time Factors

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