Dendritic cell vaccination protects mice against lethality caused by genital herpes simplex virus type 2 infection.

Abstract	We have evaluated the ability of antigen pulsed bone-marrow derived dendritic cells (bmDC), to induce protective immunity against a genital tract infection with herpes simplex virus type 2 (HSV-2) in mice. Intravenous but not vaginal administrations of bmDC pulsed in vitro with UV-inactivated HSV-2, or with purified HSV-2 envelope glycoproteins gave rise to complete protection against disease, as well as death caused by genital herpes infection. Protection was dependent on the antigens being presented by the bmDC as neither the antigens alone, nor the mock-pulsed bmDC prevented disease. Immunity was associated with HSV-2 specific IFN-gamma and antibody production, and was shown to be dependent on CD4(+) cells secreting IFN-gamma. Thus, ex vivo antigen-pulsed bmDC represents a powerful tool for the study of protective immunity to genital herpes infection, and for the identification of protective antigens. These findings might also have an impact on the design of vaccines against other sexually transmitted viral diseases.
Authors	E Schön, A M Harandi, I Nordström, J Holmgren, K Eriksson
Journal	Journal of reproductive immunology (J Reprod Immunol) Vol. 50 Issue 2 Pg. 87-104 (May 2001) ISSN: 0165-0378 [Print] Ireland
PMID	11334992 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antigens, Viral Chromium Radioisotopes Interferon-gamma
Topics	Administration, Intravaginal Animals Antigen Presentation Antigens, Viral (administration & dosage, genetics) CD4-Positive T-Lymphocytes (immunology) CHO Cells Cell Movement Chromium Radioisotopes Cricetinae Dendritic Cells (immunology, transplantation) Female Herpes Genitalis (immunology, prevention & control) Herpesvirus 2, Human (genetics, immunology, pathogenicity) In Vitro Techniques Injections, Intravenous Injections, Subcutaneous Interferon-gamma (biosynthesis, genetics) Kinetics Mice Mice, Inbred C57BL Mice, Knockout Tissue Distribution Transplantation, Isogeneic Vaccination

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