Abstract |
Hexylester of 5-aminolevulinic acid (hALA) has been considered as an alternative to 5-aminolevulinic acid (ALA) for the treatment of malignancies of different origin. The present study addresses the ALA and hALA-induced PpIX pharmacokinetic profile using rat bladder carcinoma cells (AY27). The total PpIX content measured spectrofluorimetrically after extraction procedure at the end of 2 h incubation was at least 1.5-fold greater with hALA compared to ALA despite the difference in concentration of several orders between the two compounds (1 or 5 mM ALA and 5 or 10 x 10(-3) mM hALA). Considerable PpIX efflux was detected in the extracellular medium at the end of the incubation. With 5 mM ALA and 10 x 10(-3) mM hALA, PpIX build-up was continued beyond the incubation period pointing out to enzyme saturation in the biosynthetic pathway or/and the constitution of ALA reserve. Red laser light (lambda=630 nm) irradiation of AY27 cells after 2 h incubation with increasing ALA or hALA concentrations resulted in a nearly equal photocytotoxicity.
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Authors | I Cosserat-Gerardin, L Bezdetnaya, D Notter, C Vigneron, F Guillemin |
Journal | Journal of photochemistry and photobiology. B, Biology
(J Photochem Photobiol B)
Vol. 59
Issue 1-3
Pg. 72-9
(Dec 2000)
ISSN: 1011-1344 [Print] Switzerland |
PMID | 11332893
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cell Extracts
- Culture Media
- Culture Media, Serum-Free
- Photosensitizing Agents
- Protoporphyrins
- Aminolevulinic Acid
- protoporphyrin IX
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Topics |
- Aminolevulinic Acid
(pharmacology)
- Animals
- Cell Extracts
- Cell Survival
- Culture Media
- Culture Media, Serum-Free
- Dose-Response Relationship, Drug
- Fluorescence
- Photochemotherapy
(methods)
- Photosensitizing Agents
(pharmacokinetics, pharmacology)
- Protoporphyrins
(biosynthesis, pharmacokinetics, pharmacology)
- Rats
- Tumor Cells, Cultured
- Urinary Bladder Neoplasms
(drug therapy)
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