Abstract | AIM: METHODS: Experimental atherosclerotic rabbits created by damaging the abdominal aortic endothelium and feeding with high fat diet for 8 wk were then treated with lipanthyl 15 mg.kg-1.d-1 for 16 wk. Expression of endothelin (ET)-1 mRNA and nitric oxide synthase (NOS) mRNA in atherosclerotic vessel wall was measured by in situ hybridization and reverse transcription polymerase chain reaction (RT-PCR), respectively. RESULTS: After lipanthyl administration for 16 wk, ET-1 mRNA expression was reduced, and integral optical density (IOD) and area of hybridization granule were observed to be (49,113 +/- 16,868) and (2448 +/- 621) micron 2 in lipanthyl group and (65,188 +/- 10,113) and (3028 +/- 352) micron 2 in atherosclerotic group, respectively. Regarding inducible NOS mRNA expression, IOD and area were decreased by 25.5% and 53.3%, respectively, whereas endothelial NOS mRNA expression was increased. CONCLUSION:
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Authors | M L Xie, Z L Gu, K J Chen, W X Zhou, C Y Guo |
Journal | Acta pharmacologica Sinica
(Acta Pharmacol Sin)
Vol. 21
Issue 5
Pg. 473-6
(May 2000)
ISSN: 1671-4083 [Print] United States |
PMID | 11324450
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Endothelin-1
- Hypolipidemic Agents
- RNA, Messenger
- Nitric Oxide Synthase
- Nitric Oxide Synthase Type II
- Fenofibrate
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Topics |
- Animals
- Aorta, Abdominal
(metabolism)
- Arteriosclerosis
(metabolism)
- Endothelin-1
(biosynthesis, genetics)
- Fenofibrate
(pharmacology)
- Hypolipidemic Agents
(pharmacology)
- Male
- Nitric Oxide Synthase
(biosynthesis, genetics)
- Nitric Oxide Synthase Type II
- RNA, Messenger
(biosynthesis, genetics)
- Rabbits
- Random Allocation
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