Abstract |
An expression sequence tag identified in a screen for genes upregulated by retinoic acid induced neuronal differentiation of the human teratocarcinoma cell line Ntera2/D1 was found in close genomic proximity to a region of high sequence homology to the septin subfamily of GTPase genes. We could show that the tag corresponds to the 3' untranslated region of this novel gene named septin 3 and cloned three isoforms A (2191 bp), B (4378 bp), and C (1896 bp) from human Ntera2/D1 cDNA. We present the genomic localization and organization on chromosome 22q13.2, a chromosomal hot spot for translocations implicated in leukemia. Interestingly, MSF the closest paralog of septin 3 is a fusion partner in a therapy-related acute myeloid leukemia. Quantitative PCR confirmed the upregulation of the putative septin by neuronal differentiation and northern blotting showed only one band corresponding to sep3B with a neurospecific expression pattern in adult human tissues.
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Authors | A Methner, F Leypoldt, P Joost, J Lewerenz |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 283
Issue 1
Pg. 48-56
(Apr 27 2001)
ISSN: 0006-291X [Print] United States |
PMID | 11322766
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2001 Academic Press. |
Chemical References |
- Nerve Tissue Proteins
- Protein Isoforms
- Tretinoin
- GTP Phosphohydrolases
- Septins
- septin 3
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Topics |
- Cell Differentiation
(drug effects, genetics)
- Chromosomes, Human, Pair 22
(genetics)
- Cloning, Molecular
- GTP Phosphohydrolases
(genetics, metabolism)
- Humans
- Molecular Sequence Data
- Nerve Tissue Proteins
(genetics, metabolism)
- Neurons
(cytology, metabolism)
- Organ Specificity
- Phylogeny
- Physical Chromosome Mapping
- Protein Isoforms
(genetics, metabolism)
- Septins
- Sequence Homology, Amino Acid
- Teratocarcinoma
(genetics, metabolism)
- Tretinoin
(pharmacology)
- Tumor Cells, Cultured
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