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Human septin 3 on chromosome 22q13.2 is upregulated by neuronal differentiation.

Abstract
An expression sequence tag identified in a screen for genes upregulated by retinoic acid induced neuronal differentiation of the human teratocarcinoma cell line Ntera2/D1 was found in close genomic proximity to a region of high sequence homology to the septin subfamily of GTPase genes. We could show that the tag corresponds to the 3' untranslated region of this novel gene named septin 3 and cloned three isoforms A (2191 bp), B (4378 bp), and C (1896 bp) from human Ntera2/D1 cDNA. We present the genomic localization and organization on chromosome 22q13.2, a chromosomal hot spot for translocations implicated in leukemia. Interestingly, MSF the closest paralog of septin 3 is a fusion partner in a therapy-related acute myeloid leukemia. Quantitative PCR confirmed the upregulation of the putative septin by neuronal differentiation and northern blotting showed only one band corresponding to sep3B with a neurospecific expression pattern in adult human tissues.
AuthorsA Methner, F Leypoldt, P Joost, J Lewerenz
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 283 Issue 1 Pg. 48-56 (Apr 27 2001) ISSN: 0006-291X [Print] United States
PMID11322766 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright 2001 Academic Press.
Chemical References
  • Nerve Tissue Proteins
  • Protein Isoforms
  • Tretinoin
  • GTP Phosphohydrolases
  • Septins
  • septin 3
Topics
  • Cell Differentiation (drug effects, genetics)
  • Chromosomes, Human, Pair 22 (genetics)
  • Cloning, Molecular
  • GTP Phosphohydrolases (genetics, metabolism)
  • Humans
  • Molecular Sequence Data
  • Nerve Tissue Proteins (genetics, metabolism)
  • Neurons (cytology, metabolism)
  • Organ Specificity
  • Phylogeny
  • Physical Chromosome Mapping
  • Protein Isoforms (genetics, metabolism)
  • Septins
  • Sequence Homology, Amino Acid
  • Teratocarcinoma (genetics, metabolism)
  • Tretinoin (pharmacology)
  • Tumor Cells, Cultured

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