Drug-resistant human laryngeal carcinoma cells have increased levels of cathepsin B.

Abstract

In our previous work we showed that the drug-resistance of cervical carcinoma, laryngeal carcinoma and glioblastoma cells may be accompanied by increased levels of tumor markers for invasion and metastasis (i.e. urokinase-type plasminogen activator, plasminogen activator inhibitor type 1, and/or cathepsin D). In the present study we examined the concentration of cathepsins B, L and H in three drug-resistant clones isolated from human laryngeal carcinoma (HEp2). The basal levels of cathepsins B, L and H were determined by enzyme linked immunoabsorbent assay (ELISA). Our results showed that all three clones had an increased level of cathepsin B (in two clones an almost 4-fold increase was determined). The level of cathepsin L was altered (increased) only in VK2 clone, while the levels of cathepsin H were similar in parental cells and drug-resistant clones. Thus, our results suggest that drug-resistance may be accompanied by an increased level of cathepsin B, i.e. tumor associated protease, involved in invasion and metastasis.

• Authors: M Osmak, B Svetić, D Gabrijelcić-Geiger, J Skrk
• Journal: Anticancer research (Anticancer Res) 2001 Jan-Feb Vol. 21 Issue 1A Pg. 481-3 ISSN: 0250-7005 [Print] Greece
• PMID: 11299783 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Cathepsins
• Endopeptidases
• Cysteine Endopeptidases
• Cathepsin B
• CTSL protein, human
• Cathepsin L
• CTSH protein, human
• Cathepsin H

Topics

• Biomarkers, Tumor (metabolism)
• Carcinoma (drug therapy, metabolism)
• Cathepsin B (metabolism)
• Cathepsin H
• Cathepsin L
• Cathepsins (metabolism)
• Clone Cells
• Cysteine Endopeptidases (metabolism)
• Drug Resistance, Neoplasm
• Endopeptidases
• Humans
• Laryngeal Neoplasms (drug therapy, metabolism)
• Prognosis
• Tumor Cells, Cultured