Cytotoxicity effects of di- and tri-hydroxybenzaldehydes as a chemopreventive potential agent on tumor cells.

Abstract

As part of our earlier search for new compounds with improved biological activities including antioxidant, anti-inflammatory, and tumor growth inhibition activities, we synthesized 2,4,5-trihydroxybenzaldehyde (2,4,5-THBA) from commercially available Sesamol. First we examined the free radical-quenching capacity of 2,4,5-THBA, 3,4-dihydroxybenzaldehyde (3,4-DHBA), and 2,5-dihydroxybenzaldehyde (2,5-DHBA) in vitro by 1,1-diphenyl-1,2-picryhydrazylradical (DPPH) test. The antioxidant bioactivity was also evaluated using the model of t-butyl hydroperoxide (t-BHP)-induced cytotoxicity in rat primary hepatocytes. Finally, three chemicals were tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay on human nasopharynx carcinoma cells (KB cells), human hepatoblastoma (HepG2), and human leukemia HL-60 cells. The 2,4,5-THBA shows significant cytotoxicity on HL-60 cells. The results suggest that 2,4,5-THBA may be a potential chemopreventor or chemotherapy agent against HL-60 cells.

• Authors: T H Tseng, Y M Tsheng, Y J Lee
• Journal: Toxicology (Toxicology) Vol. 161 Issue 3 Pg. 179-87 (Mar 28 2001) ISSN: 0300-483X [Print] Ireland
• PMID: 11297806 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anticarcinogenic Agents
• Antioxidants
• Benzaldehydes
• Formazans
• Free Radicals
• Tetrazolium Salts
• MTT formazan

Topics

• Animals
• Anticarcinogenic Agents (chemical synthesis, pharmacology, toxicity)
• Antioxidants (chemical synthesis, pharmacology)
• Benzaldehydes (chemical synthesis, pharmacology, toxicity)
• Formazans (chemistry)
• Free Radicals (chemistry)
• HL-60 Cells (drug effects)
• Hepatocytes (drug effects)
• Humans
• KB Cells (drug effects)
• Male
• Rats
• Rats, Sprague-Dawley
• Tetrazolium Salts (chemistry)