The aim of the present study was to determine the relationships between
bone morphogenetic proteins (BMPs),
BMP receptor type IA and
carcinogenesis of oral epithelium. A retrospective study was performed on material obtained from oral mucosa, including nine cases of normal mucosa (NB), eight cases of nonspecific chronic
inflammation (NCI), seven cases of hyperkeratosis (HK), five cases of
squamous cell papilloma (SCP), 29 cases of
squamous cell carcinoma (SCC) with various grades of differentiation and 10 cases of epithelium adjacent to
carcinoma (EAC). Six cases of NB from hard palate (NHP) were chosen as a control group. The benign groups consisted of NCI, HK and SCP. The
antibodies against BMP-2/4, -5, receptor BMPR-IA and purified bovine BMP (bBMP-McAb) were utilised using an immunocytochemical method. The results demonstrated that the immunostaining of BMP-2/4,
BMP-5, BMPR-IA and bBMP-McAb was weak and not consistent in normal and benign groups. The immunoreactivity level was independent of the clinical and pathological grading of SCC. All cases of SCC showed positive staining for BMP-2/4,
BMP-5, BMPR-IA and bBMP-McAb except for three cases and one case of SCC which negatively stained for BMP-2/4 and
BMP-5, respectively. The staining intensity and proportion of the positively stained cells were markedly increased in SCC when compared with that of the normal and benign groups except for EAC. The metastatic
carcinoma cells in lymph nodes were strongly and positively stained for BMP-2/4 and
BMP-5 when compared with the primary lesions. Our results indicate that there was an overexpression of BMP-2/4,
BMP-5, bBMP-McAb and BMPR-IA in the high-risk premalignant and malignant lesions of oral epithelium. Our findings suggest that BMP-2/4 and
BMP-5 but not BMPR-IA might be involved in the
metastasis of oral
carcinoma cells.