HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Nasal or intramuscular immunization of mice with influenza subunit antigen and the B subunit of Escherichia coli heat-labile toxin induces IgA- or IgG-mediated protective mucosal immunity.

Abstract
Local mucosal IgA antibodies play a central role in protection of the respiratory tract against influenza virus infection. Therefore, new-generation influenza vaccines should aim at stimulating not only systemic, but also local antibody responses. Previously, we demonstrated that the recombinant B subunit of the Escherichia coli heat-labile toxin (LTB) is a potent adjuvant towards nasally administered influenza subunit antigen. Here, we investigated the protection conferred by LTB-supplemented influenza subunit antigen given intranasally (i.n.) or intramuscularly (i.m.) to mice. Both i.n. and i.m. immunization with subunit antigen and LTB completely protected the animals against viral infection. Protection upon i.n. immunization was associated with the induction of antigen-specific serum IgG and mucosal IgA, whereas protection upon i.m. immunization correlated with strong serum and mucosal IgG, but not IgA responses. We conclude that LTB-supplemented influenza subunit antigen, given either i.n. or i.m, induces protective antibody-mediated mucosal immunity and thus represents a promising novel flu vaccine candidate.
AuthorsL Haan, W R Verweij, M Holtrop, R Brands, G J van Scharrenburg, A M Palache, E Agsteribbe, J Wilschut
JournalVaccine (Vaccine) Vol. 19 Issue 20-22 Pg. 2898-907 (Apr 06 2001) ISSN: 0264-410X [Print] Netherlands
PMID11282201 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adjuvants, Immunologic
  • Antibodies, Viral
  • Bacterial Toxins
  • Enterotoxins
  • Escherichia coli Proteins
  • Immunoglobulin A, Secretory
  • Immunoglobulin G
  • Influenza Vaccines
  • Protein Subunits
  • heat-labile enterotoxin, E coli
Topics
  • Adjuvants, Immunologic (administration & dosage)
  • Administration, Intranasal
  • Animals
  • Antibodies, Viral (immunology)
  • Bacterial Toxins (administration & dosage)
  • Enterotoxins (administration & dosage)
  • Escherichia coli Proteins
  • Female
  • Immunization
  • Immunoglobulin A, Secretory (immunology)
  • Immunoglobulin G (immunology)
  • Influenza Vaccines (administration & dosage, immunology)
  • Injections, Intramuscular
  • Lung (virology)
  • Mice
  • Mice, Inbred BALB C
  • Nasal Mucosa (virology)
  • Protein Subunits
  • Rats

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: