Abstract | OBJECTIVES: METHODS: In this pilot study, IgG was isolated from the sera of women with advanced-stage ovarian cancer (stages III and IV, n = 62) and age-matched female volunteers (n = 50) by affinity chromatography. These IgGs were characterized on the basis on their aberrant binding to concanavalin A affinity columns. Subsequently, the concanavalin A-binding moiety was localized following IgG fragmentation, analyzed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis, and characterized by oligosaccharide profiling. RESULTS: CONCLUSIONS: While our previous studies have identified the presence of circulating IgG reactive with specific tumor-associated antigens and its association with poor prognosis, this report demonstrated the presence of an aberrantly glycosylated IgG population in cancer patients. This altered IgG appeared to be the primary class of tumor-reactive antibodies in these women.
|
Authors | C Gerçel-Taylor, L B Bazzett, D D Taylor |
Journal | Gynecologic oncology
(Gynecol Oncol)
Vol. 81
Issue 1
Pg. 71-6
(Apr 2001)
ISSN: 0090-8258 [Print] United States |
PMID | 11277653
(Publication Type: Journal Article)
|
Copyright | Copyright 2001 Academic Press. |
Chemical References |
- Antibodies, Neoplasm
- Antigens, Neoplasm
- Immunoglobulin G
- Oligosaccharides
- Receptors, Concanavalin A
- Concanavalin A
|
Topics |
- Antibodies, Neoplasm
(blood, immunology)
- Antigens, Neoplasm
(immunology)
- Chromatography, Affinity
- Concanavalin A
(immunology, metabolism)
- Female
- Humans
- Immunoglobulin G
(blood, immunology, isolation & purification)
- Middle Aged
- Neoplastic Cells, Circulating
(immunology)
- Oligosaccharides
(immunology, metabolism)
- Ovarian Neoplasms
(blood, immunology, pathology)
- Pilot Projects
- Receptors, Concanavalin A
(immunology, metabolism)
|