Abstract | BACKGROUND AND OBJECTIVES: METHODS: Fluorescence in situ hybridization was performed to examine changes in chromosomal numbers in a total of 13 pancreatic cancer cell lines. We also assessed the potential for tumor aggressiveness within cancer cells by in vitro migration and invasion assay and by subcutaneous implantation into nude mice. RESULTS:
Chromosomal instability, characterized by numerical variations in copy numbers of chromosome 8, was observed in most cell lines, and the magnitude of instability was correlated well with both motility (P < 0.001) and invasion rate (P < 0.001) of these cells. Furthermore, a significant positive correlation existed between chromosome instability and tumor growth in vivo (P < 0.01). CONCLUSIONS:
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Authors | N Sato, K Mizumoto, N Maehara, M Shono, E Nagai, M Tanaka |
Journal | Journal of surgical oncology
(J Surg Oncol)
Vol. 76
Issue 3
Pg. 181-7
(Mar 2001)
ISSN: 0022-4790 [Print] United States |
PMID | 11276022
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2001 Wiley-Liss, Inc. |
Topics |
- Animals
- Cell Count
- Cell Movement
- Chromosome Aberrations
- Chromosomes, Human, Pair 8
(genetics)
- Humans
- In Situ Hybridization, Fluorescence
- Mice
- Mice, Nude
- Neoplasm Invasiveness
- Pancreatic Neoplasms
(genetics, pathology)
- Tumor Cells, Cultured
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