Abstract | BACKGROUND AND AIMS: METHODS: RESULTS: The PLA2 inhibitor FPL 67047XX retarded cholesterol absorption in a lymph fistula rat model. Under basal chow-fed dietary conditions, cholesterol absorption efficiency from a single bolus meal, and plasma lipid levels, were similar among PLA2+/+, PLA2+/-, and PLA2-/- mice. Interestingly, the nonhydrolyzable phospholipid dioleoyl ether phosphatidylcholine suppressed cholesterol absorption by 10% to 18% in mice without regard to their PLA2 genotype. When 1-palmitoyl-2-[(14)C]oleoyl- phosphatidylcholine was used as the substrate, the radiolabeled phospholipid was found to be hydrolyzed and absorbed with equal efficiency in PLA2+/+, PLA2+/-, and PLA2-/- mice. CONCLUSIONS: These results suggested that although phospholipid digestion in the intestinal lumen is a prerequisite for efficient cholesterol absorption, additional enzyme(s) can compensate for pancreatic PLA2 in catalyzing phospholipid digestion and facilitating cholesterol absorption in PLA2 knockout mice.
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Authors | B L Richmond, A C Boileau, S Zheng, K W Huggins, N A Granholm, P Tso, D Y Hui |
Journal | Gastroenterology
(Gastroenterology)
Vol. 120
Issue 5
Pg. 1193-202
(Apr 2001)
ISSN: 0016-5085 [Print] United States |
PMID | 11266383
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Carbon Radioisotopes
- Cholesterol, Dietary
- Triglycerides
- Phospholipases A
- Phospholipases A2
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Topics |
- Animals
- Carbon Radioisotopes
- Cholesterol, Dietary
(pharmacokinetics)
- Digestive System Fistula
(metabolism)
- Female
- Intestinal Absorption
(physiology)
- Lymph
(metabolism)
- Male
- Mice
- Mice, Inbred C3H
- Mice, Inbred C57BL
- Mice, Knockout
- Phospholipases A
(genetics, metabolism)
- Phospholipases A2
- Pregnancy
- Rats
- Rats, Sprague-Dawley
- Triglycerides
(pharmacokinetics)
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