Influence of batroxobin on cerebral ischemia-reperfusion injury in gerbils.

Abstract

AIM:

To study the effects of batroxobin (Bat) on neurons survival, neurobehavioral test, ATP levels and hydroxyl radical outputs in hippocampus during forebrain ischemia-reperfusion in gerbils.

METHODS:

The forebrain ischemia was induced by occluding the bilateral common carotid arteries for 10 min in gerbils, and ATP levels and 2, 3-dihydroxybenzoic acid (DHBA) outputs were assayed by HPLC. The neurons survival were assessed by histology, and behavioral tests of gerbils were assessed by open field test.

RESULTS:

The number of neurons survival in Ir at d 7 postischemic insult were (7 +/- 4)% of sham-operated gerbils, much less than that in Bat (45 +/- 16)%. The levels of explore activities of ischemic gerbils was 175% and 159% of sham-operated gerbils at d 3 and d 6 postischemic insult, much more than that in Bat (120% d 3 and 140% d 6). Hippocampal ATP levels in Ir were 64% of sham-operated gerbils at reperfusion 60 min, much less than that in Bat I and II (82% and 89% respectively). The hippocampal 2,3-DHBA outputs in Ir increased by 4.5 folds of sham-operated gerbils at reperfusion 60 min, but the 2,3-DHBA outputs in Bat I and Bat II were only 2.6 and 2.4 folds respectively.

CONCLUSION:

Bat possesses the inhibitory effects on DND and OH. production following cerebral ischemia-reperfusion in gerbils.

• Authors: Q Chen, Y M Zeng, P C Xu, J W Fan
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 21 Issue 2 Pg. 161-4 (Feb 2000) ISSN: 1671-4083 [Print] United States
• PMID: 11263264 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fibrinolytic Agents
• Hydroxybenzoates
• 2,3-dihydroxybenzoic acid
• Adenosine Triphosphate
• Batroxobin

Topics

• Adenosine Triphosphate (metabolism)
• Animals
• Batroxobin (pharmacology)
• Brain Ischemia (complications)
• Cell Survival (drug effects)
• Fibrinolytic Agents (pharmacology)
• Gerbillinae
• Hippocampus (metabolism, pathology)
• Hydroxybenzoates (metabolism)
• Male
• Maze Learning
• Neurons (drug effects)
• Reperfusion Injury (etiology, metabolism, pathology)