Expressions of proliferation markers (Ki-67, proliferating cell nuclear antigen, and silver-staining nucleolar organizer regions) and of p53 tumor protein in gestational trophoblastic disease.

Abstract

OBJECTIVE:

This study was undertaken to determine whether the expressions of 3 proliferation markers (Ki-67, proliferating cell nuclear antigen, and silver-staining nucleolar organizer regions) and of p53 tumor protein could differentiate spontaneous abortions from gestational trophoblastic diseases and also discriminate among gestational trophoblastic disease subgroups.

STUDY DESIGN:

Twenty-two partial hydatidiform moles, 17 complete hydatidiform moles, 6 invasive hydatidiform moles, and 20 nonhydropic spontaneous abortions (control group) were evaluated by means of immunohistochemical techniques with antibodies to Ki-67, proliferating cell nuclear antigen, and p53. One-step silver staining was used to detect silver-staining nucleolar organizer regions.

RESULTS:

The expressions of Ki-67, proliferating cell nuclear antigen, silver-staining nucleolar organizer regions, and p53 were significantly higher in the gestational trophoblastic disease group than in the control group. The results of linear discriminant analysis showed that silver-staining nucleolar organizer region count had the highest sensitivity and specificity (93.3% and 100%, respectively) for distinguishing gestational trophoblastic disease from spontaneous abortion. Sensitivity and specificity for discriminating gestational trophoblastic disease from spontaneous abortion increased to 100% when all four markers were used together. Proliferating cell nuclear antigen was found to be the best discriminating variable for differentiating among gestational trophoblastic disease subgroups.

CONCLUSION:

Our findings suggest that expressions of Ki-67, proliferating cell nuclear antigen, silver-staining nucleolar organizer regions, and p53 may aid in the diagnosis of gestational trophoblastic diseases. These fairly rapid, simple, and economic techniques could serve as a useful adjunct to conventional methods in the diagnosis of gestational trophoblastic diseases.

• Authors: A Kale, F Söylemez, A Ensari
• Journal: American journal of obstetrics and gynecology (Am J Obstet Gynecol) Vol. 184 Issue 4 Pg. 567-74 (Mar 2001) ISSN: 0002-9378 [Print] United States
• PMID: 11262454 (Publication Type: Journal Article)

Chemical References

• Ki-67 Antigen
• Proliferating Cell Nuclear Antigen
• Tumor Suppressor Protein p53

Topics

• Abortion, Spontaneous (pathology)
• Adolescent
• Adult
• Cell Division
• Cell Nucleus (chemistry)
• Female
• Humans
• Hydatidiform Mole (immunology, pathology)
• Hydatidiform Mole, Invasive (immunology, pathology)
• Immunohistochemistry
• Ki-67 Antigen (analysis)
• Nucleolus Organizer Region (ultrastructure)
• Pregnancy
• Proliferating Cell Nuclear Antigen (analysis)
• Sensitivity and Specificity
• Silver Staining
• Trophoblasts (pathology)
• Tumor Suppressor Protein p53 (analysis)
• Uterine Neoplasms (immunology, pathology)