Lack of HLA-class I antigens in human neuroblastoma cells: analysis of its relationship to TAP and tapasin expression.

We studied the constitutive and the interferon (IFN)-gamma-induced expression of HLA class I antigen heavy chain, beta2-microglobulin (beta2m), TAP-1, TAP-2 and tapasin in a panel of eleven neuroblastoma cell lines. Surface expression of HLA class I antigens was low in eight out of eight neuroblastoma cell lines bearing MYC-N amplification and/or 1p deletion, while two out of three neuroblastoma cell lines lacking these genetic alterations showed normal expression. IFN-gamma treatment restored HLA class I antigen surface expression in all neuroblastoma cell lines. Eight out of 11 neuroblastoma cell lines did not express TAP-1 mRNA and three of them also lacked TAP-2 mRNA. beta2 m mRNA was barely detectable or absent in five neuroblastoma cell lines, while tapasin mRNA was always expressed. IFN-gamma upregulated the expression of HLA class I heavy chain, beta2 m, TAP-1, TAP-2 and tapasin, as detected at mRNA or protein level. Post-transcriptional events were involved in altered TAP-1 and beta2 m expression in one peculiar neuroblastoma cell line. These data indicate that multiple mechanisms play a role in the HLA class I antigen-deficient phenotype of human neuroblastoma.
AuthorsM V Corrias, M Occhino, M Croce, A De Ambrosis, M P Pistillo, P Bocca, V Pistoia, S Ferrini
JournalTissue antigens (Tissue Antigens) Vol. 57 Issue 2 Pg. 110-7 (Feb 2001) ISSN: 0001-2815 [Print] Denmark
PMID11260505 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Surface
  • Antineoplastic Agents
  • Antiporters
  • Extracellular Matrix Proteins
  • Histocompatibility Antigens Class I
  • Immunoglobulin Heavy Chains
  • Immunoglobulins
  • Membrane Transport Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • TAP1 protein, human
  • beta 2-Microglobulin
  • tapasin
  • TAP2 protein, human
  • Interferon-gamma
  • ATP-Binding Cassette Transporters (analysis, genetics, immunology)
  • Antigens, Surface (genetics, immunology)
  • Antineoplastic Agents (pharmacology)
  • Antiporters (analysis, genetics, immunology)
  • Blotting, Western
  • Brain Neoplasms (immunology)
  • Extracellular Matrix Proteins (analysis, genetics, immunology)
  • Gene Deletion
  • Gene Expression (drug effects, immunology)
  • Genes, myc
  • Histocompatibility Antigens Class I (analysis, genetics, immunology)
  • Humans
  • Immunoglobulin Heavy Chains (analysis, genetics, immunology)
  • Immunoglobulins (analysis, genetics, immunology)
  • Interferon-gamma (pharmacology)
  • Membrane Transport Proteins
  • Nerve Tissue Proteins (analysis, genetics, immunology)
  • Neuroblastoma (immunology)
  • RNA, Messenger (analysis)
  • Tumor Cells, Cultured
  • beta 2-Microglobulin (analysis, genetics, immunology)

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