Abstract |
Infection of dengue viruses (DENs) can cause human dengue fever, hemorrhagic fever, or shock syndrome. Although DEN-induced apoptosis has been implicated in pathogenesis of the DEN-related diseases, the underlying mechanism remains largely unexplored. In this study, we investigated the effect of ectopic expression of human bcl-2 and bcl-X genes on DEN-induced apoptosis in cultured cells. We employed a human isolate of DEN serotype 2 (DEN-2), PL046, which not only caused cell-cycle arrest in the G1 phase but also induced apoptosis in infected baby hamster kidney (BHK-21) cells, murine neuroblastoma N18 cells, and human neuronal NT-2 cells. Our results reveal that overexpression of bcl-2 in fibroblast-like BHK-21 cells, although not inhibiting virus yields, delayed the process of DEN-induced apoptosis, thereby permitting surviving cells to become persistently infected. In contrast, stable bcl-2 expression in neuronal N18 cells failed to block DEN-induced apoptosis. On the other hand, Bcl-X(L), expressed predominantly in the nervous system, appeared to delay DEN's killing effect in neuronal N18 cells but not in fibroblast-like BHK-21 cells. In addition, inducible expression bcl-X(s), despite its proapoptotic property in other reported system, was found to merely accelerate cell death in DEN-infected N18 but not in infected BHK-21 cells. Thus, through studying the effect of human bcl-2-related genes, our results suggest that DEN infection may trigger target cells to undergo morphologically similar but biochemically distinct apoptotic pathways in a cell-specific manner.
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Authors | H L Su, Y L Lin, H P Yu, C H Tsao, L K Chen, Y T Liu, C L Liao |
Journal | Virology
(Virology)
Vol. 282
Issue 1
Pg. 141-53
(Mar 30 2001)
ISSN: 0042-6822 [Print] United States |
PMID | 11259197
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright 2001 Academic Press. |
Chemical References |
- BCL2L1 protein, human
- Bcl2l1 protein, mouse
- Proto-Oncogene Proteins c-bcl-2
- bcl-X Protein
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Topics |
- Animals
- Apoptosis
- Cell Survival
- Cells, Cultured
- Dengue Virus
- G1 Phase
- Genes, bcl-2
(genetics)
- Humans
- Mice
- Neurons
(virology)
- Proto-Oncogene Proteins c-bcl-2
(genetics, metabolism)
- Transfection
- Virus Replication
- bcl-X Protein
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