Abstract |
Individuals affected by the autosomal recessive disorder Werner's syndrome (WS) develop many of the symptoms characteristic of premature ageing. Primary fibroblasts cultured from WS patients exhibit karyotypic abnormalities and a reduced replicative life span. The WRN gene encodes a 3'-5' DNA helicase, and is a member of the RecQ family, which also includes the product of the Bloom's syndrome gene (BLM). In this work, we show that WRN promotes the ATP-dependent translocation of Holliday junctions, an activity that is also exhibited by BLM. In cells arrested in S-phase with hydroxyurea, WRN localizes to discrete nuclear foci that coincide with those formed by the single-stranded DNA binding protein replication protein A. These results are consistent with a model in which WRN prevents aberrant recombination events at sites of stalled replication forks by dissociating recombination intermediates.
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Authors | A Constantinou, M Tarsounas, J K Karow, R M Brosh, V A Bohr, I D Hickson, S C West |
Journal | EMBO reports
(EMBO Rep)
Vol. 1
Issue 1
Pg. 80-4
(Jul 2000)
ISSN: 1469-221X [Print] England |
PMID | 11256630
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Proteins
- Recombinant Proteins
- Adenosine Triphosphate
- DNA
- Exodeoxyribonucleases
- Adenosine Triphosphatases
- Bloom syndrome protein
- DNA Helicases
- RecQ Helicases
- WRN protein, human
- Werner Syndrome Helicase
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Topics |
- Adenosine Triphosphatases
(genetics, metabolism)
- Adenosine Triphosphate
(metabolism)
- Bacterial Proteins
(genetics, metabolism)
- Cell Nucleus
(metabolism)
- DNA
(genetics, metabolism)
- DNA Helicases
(genetics, metabolism)
- DNA Replication
- Exodeoxyribonucleases
- HeLa Cells
- Humans
- Microscopy, Fluorescence
- RecQ Helicases
- Recombinant Proteins
(genetics, metabolism)
- Recombination, Genetic
- Werner Syndrome
(genetics, metabolism)
- Werner Syndrome Helicase
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