Abstract |
Gamma-thujaplicin and beta-dolabrin, the constituents of the wood of Thujopsis dolabrata Sieb. et Zucc. var. hondai showed strong in vitro cytotoxic effects against the human stomach cancer cell lines KATO-III and Ehrlich's ascites carcinoma. The cytotoxic effects of the two compounds against both tumor cell lines were clear when cell growth was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Gamma-thujaplicin and beta-dolabrin at 0.32 microg/ml inhibited cell growth of human stomach cancer KATO-III by 85 and 67%, and Ehrlich's ascites carcinoma by 91 and 75%, respectively. There is no large difference in cytotoxicity between these compounds, but the activity of gamma-thujaplicin was slightly more potent than that of beta-dolabrin. On the other hand, hinokitiol acetate did not show a cytotoxic effect, suggesting that at least a part of the mechanism of the cytotoxic effect of hinokitiol-related compounds is due to metal chelation between the carbonyl group at C-1 and the hydroxyl group at C-2 in the tropolone skeleton of these molecules. The acute toxicities [50% lethal dose (LD50) value: intraperitoneal injection, Van der Waedem] of gamma-thujaplicin and beta-dolabrin in mice were 277 mg/kg and 232 mg/kg, respectively.
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Authors | E Matsumura, Y Morita, T Date, H Tsujibo, M Yasuda, T Okabe, N Ishida, Y Inamori |
Journal | Biological & pharmaceutical bulletin
(Biol Pharm Bull)
Vol. 24
Issue 3
Pg. 299-302
(Mar 2001)
ISSN: 0918-6158 [Print] Japan |
PMID | 11256489
(Publication Type: Journal Article)
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Chemical References |
- 2-hydroxy-5-isopropyl- 2,4,6-cycloheptatrienone
- Antineoplastic Agents, Phytogenic
- Cycloheptanes
- Monoterpenes
- beta-dolabrin
- Tropolone
- beta-thujaplicin
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Topics |
- Animals
- Antineoplastic Agents, Phytogenic
(pharmacology, toxicity)
- Body Weight
(drug effects)
- Carcinoma, Ehrlich Tumor
(pathology)
- Cycloheptanes
(pharmacology, toxicity)
- Drug Screening Assays, Antitumor
- Humans
- Lethal Dose 50
- Male
- Mice
- Monoterpenes
- Stomach Neoplasms
(pathology)
- Tropolone
(analogs & derivatives, pharmacology, toxicity)
- Tumor Cells, Cultured
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