Preferential degradation of osteoclasts by titanium tetrachloride.

Abstract

Although titanium alloys are known to be biocompatible with bone tissue after implantation in human beings, the effect of titanium on osteoclasts remains to be studied. We examined the effect of titanium salt on the formation and survival of osteoclasts in cell culture. The addition of 10 microM titanium tetrachloride caused a decrease in the cell number of osteoclast-like cells induced in bone marrow cell cultures taken from mice. The addition of 10 microM titanium tetrachloride caused degradation of the disaggregated osteoclasts taken from neonatal rats and a decrease in bone resorption. Along with the increase in the degradation of osteoclasts, the number of apoptotic cells increased. Titanium tetrachloride dose-dependently decreased the cell number and alkaline phosphatase activity of osteoblastic cell cultures taken from rat calvaria. However, these concentrations were 30-40 times higher than those in the case of osteoclast-like cell formation. These results showed that titanium ions caused a preferential degradation of osteoclasts rather than osteoblasts, most likely by apoptosis.

• Authors: T Matsunaga, T Kojo, T Tsujisawa, T Fukuizumi, S Wada, Y Uchida, H Inoue
• Journal: Journal of biomedical materials research (J Biomed Mater Res) Vol. 55 Issue 3 Pg. 313-9 (Jun 05 2001) ISSN: 0021-9304 [Print] United States
• PMID: 11255184 (Publication Type: Journal Article)
• Copyright: Copyright 2001 John Wiley & Sons, Inc. J Biomed Mater Res 55: 313-319, 2001

Chemical References

• Biocompatible Materials
• titanium tetrachloride
• Titanium
• Alkaline Phosphatase

Topics

• Alkaline Phosphatase (metabolism)
• Animals
• Apoptosis (drug effects)
• Biocompatible Materials (toxicity)
• Bone Resorption (pathology, prevention & control)
• Cell Division (drug effects)
• Humans
• In Vitro Techniques
• Materials Testing
• Mice
• Mice, Inbred ICR
• Osteoclasts (cytology, drug effects, enzymology)
• Titanium (toxicity)