Accumulation of barley stripe mosaic virus is significantly reduced in transgenic wheat plants expressing a bacterial ribonuclease.

Abstract

An rnc70 gene encoding a mutant bacterial ribonuclease III (RNase III) was introduced into wheat (Triticum aestivum cv. Bobwhite) by microprojectile bombardment. T1, T2, and T3 plants regenerated from three transgenic callus lines were challenged with barley stripe mosaic virus. Plants expressing RNase III exhibited a high level of resistance to the virus infection. This resistance was evidenced by the absence of virus symptoms and reduced accumulation of virions in these plants. The result demonstrates that this pathogen-targeted resistance strategy can be effectively employed in conferring resistance to viral diseases of cereal crops.

• Authors: L Zhang, R French, W G Langenberg, A Mitra
• Journal: Transgenic research (Transgenic Res) Vol. 10 Issue 1 Pg. 13-9 ( 2001) ISSN: 0962-8819 [Print] Netherlands
• PMID: 11252379 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• DNA Primers
• RNA, Viral
• Endoribonucleases
• Ribonuclease III

Topics

• Blotting, Western
• Cloning, Molecular
• DNA Primers (chemistry)
• Endoribonucleases (genetics, metabolism)
• Gene Expression
• Hordeum (virology)
• Mosaic Viruses (genetics, physiology)
• Mutation
• Plants, Genetically Modified (enzymology, genetics)
• Polymerase Chain Reaction
• RNA, Viral (genetics, metabolism)
• Ribonuclease III
• Transformation, Genetic
• Triticum (enzymology, virology)
• Virus Replication