Osmotic stress causes a G1 cell cycle delay and downregulation of Cln3/Cdc28 activity in Saccharomyces cerevisiae.

Abstract	Moderate hyperosmotic stress on Saccharomyces cerevisiae cells produces a temporary delay at the G1 stage of the cell cycle. This is accompanied by transitory downregulation of CLN1, CLN2 and CLB5 transcript levels, although not of CLN3, which codes for the most upstream activator of the G1/S transition. Osmotic shock to cells synchronized in early G1, when Cln3 is the only cyclin present, causes a delay in cell cycle resumption. This points to Cln3 as being a key cell cycle target for osmotic stress. We have observed that osmotic shock causes downregulation of the kinase activity of Cln3-Cdc28 complexes. This is concomitant with a temporary accumulation of Cln3 protein as a result of increased stability. The effects of the osmotic stress in G1 are not suppressed in CLN3-1 cells with increased kinase activity, as the Cln3-Cdc28 activity in this mutant is still affected by the shock. Although Hog1 is not required for the observed cell cycle arrest in hyperosmotic conditions, it is necessary to resume the cell cycle at KCl concentrations higher than 0.4 M.
Authors	G Bellí, E Garí, M Aldea, E Herrero
Journal	Molecular microbiology (Mol Microbiol) Vol. 39 Issue 4 Pg. 1022-35 (Feb 2001) ISSN: 0950-382X [Print] England
PMID	11251821 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	CLN1 protein, S cerevisiae CLN2 protein, S cerevisiae CLN3 protein, S cerevisiae CLN3 protein, human Cyclins Fungal Proteins Membrane Glycoproteins Membrane Proteins Molecular Chaperones SBF protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Tripeptidyl-Peptidase 1 CDC28 Protein Kinase, S cerevisiae HOG1 protein, S cerevisiae Mitogen-Activated Protein Kinases
Topics	Alleles CDC28 Protein Kinase, S cerevisiae (metabolism) Cyclins (genetics, metabolism) Down-Regulation Fungal Proteins (genetics, metabolism) G1 Phase Genes, Fungal Membrane Glycoproteins Membrane Proteins (metabolism) Mitogen-Activated Protein Kinases (metabolism) Molecular Chaperones Osmotic Pressure Saccharomyces cerevisiae (cytology, growth & development, metabolism) Saccharomyces cerevisiae Proteins Transcription Factors (metabolism) Tripeptidyl-Peptidase 1

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