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Changes in cardiac contractile function and myocardial.

Abstract
Cutaneous burn trauma causes cardiac contraction and relaxation defects, but the mechanism is unclear. Previous studies suggest that burn-related changes in myocyte handling of calcium may play an important role in postburn cardiac dysfunction. With the use of a high dissociation constant (K(d)) calcium indicator 1,2-bis(2-amino-5,6-difluorophenoxy)-ethane-N,N,N',N'-tetraacetic acid (TF-BAPTA) and (19)F NMR spectroscopy, this study examined the correlation between the changes in cytosolic free calcium concentration ([Ca(2+)](i)) and cardiac function after burn trauma. Sprague-Dawley rats were given scald burn (over 40% of the total body surface area) or sham burn. Twenty-four hours later, the hearts were excised and perfused by the Langendorff method with a modified phosphate-free Krebs-Henseleit bicarbonate buffer. Left ventricular (LV) developed pressure (LVDP), calculated from peak systolic LV pressure and LV end-diastolic pressure, was assessed through a catheter attached to an intraventricular balloon. At the same time, (31)P and (19)F NMR spectroscopy was performed before and after TF-BAPTA loading. LVDP measured in hearts from burned rats was <40% than that measured in hearts from sham burn rats (65 +/- 6 vs. 110 +/- 12 mmHg, P < 0.01); [Ca(2+)](i) was increased fourfold in hearts from the burned group compared with that measured in the sham burn group (0.807 +/- 0.192 vs. 3.891 +/- 0.929 microM). Loading TF-BAPTA in hearts transiently decreased LVDP by 15%. Phosphocreatine-to-P(i) ratio decreased, but ATP and intracellular pH remained unchanged by either TF-BAPTA loading or burn trauma. In conclusion, burn trauma impaired cardiac contractility, and this functional defect was paralleled by a significant rise in [Ca(2+)](i) in the heart.
AuthorsZ F Xia, P Zhao, J W Horton
JournalAmerican journal of physiology. Heart and circulatory physiology (Am J Physiol Heart Circ Physiol) Vol. 280 Issue 4 Pg. H1916-22 (Apr 2001) ISSN: 0363-6135 [Print] United States
PMID11247809 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Chelating Agents
  • Fluorine Radioisotopes
  • 1,2-bis(2-amino-5,6-difluorophenoxy)ethane-N,N,N',N'-tetraacetic acid
  • Egtazic Acid
  • Adenosine Triphosphate
  • Calcium
Topics
  • Adenosine Triphosphate (metabolism)
  • Animals
  • Burns (metabolism, physiopathology)
  • Calcium (metabolism, pharmacology, physiology)
  • Chelating Agents (pharmacology)
  • Egtazic Acid (analogs & derivatives, pharmacology)
  • Fluorine Radioisotopes
  • Heart (drug effects, physiology, physiopathology)
  • In Vitro Techniques
  • Kinetics
  • Magnetic Resonance Spectroscopy (methods)
  • Male
  • Myocardial Contraction (drug effects, physiology)
  • Myocardium (metabolism)
  • Perfusion
  • Rats
  • Rats, Sprague-Dawley

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