Cloning and Expression of Guanylin from the European eel (Anguilla anguilla).

Abstract	Extracts of intestinal epithelia from the European eel (Anguilla anguilla) stimulated cGMP production in the T84 human colon carcinoma cell line which suggested the presence of a guanylin-like peptide in this teleost fish. Degenerate oligonucleotide primers were subsequently used in RT-PCR resulting in the amplification, cloning, and sequencing of two cDNAs which represent possible 5' spliceoforms of an eel homologue of the mammalian peptide, guanylin. Northern blotting indicated that the main site of expression of the eel peptide is in the intestine with much lower signals also detected in the kidney. Intestinal expression of guanylin mRNA is up-regulated in both nonmigratory "yellow" and the more sexually mature, migratory "silver" eels following acclimation to the seawater environment. These results suggest that this peptide signalling system may play a role in osmoregulation in euryhaline teleost fish during migration between the marine and freshwater environments.
Authors	M M Comrie, C P Cutler, G Cramb
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 281 Issue 5 Pg. 1078-85 (Mar 2001) ISSN: 0006-291X [Print] United States
PMID	11243845 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright	Copyright 2001 Academic Press.
Chemical References	Gastrointestinal Hormones Natriuretic Peptides Peptides Proteins RNA, Messenger Tissue Extracts guanylin, European eel guanylin Cyclic GMP
Topics	Acclimatization Amino Acid Sequence Anguilla (genetics) Animals Base Sequence Cloning, Molecular Cyclic GMP (biosynthesis) Gastrointestinal Hormones Humans Intestinal Mucosa (metabolism) Kidney (metabolism) Molecular Sequence Data Natriuretic Peptides Peptides (genetics, metabolism) Proteins (genetics, metabolism) RNA, Messenger (biosynthesis) Seawater Sequence Homology, Amino Acid Tissue Extracts (pharmacology) Transcription, Genetic Tumor Cells, Cultured

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