Abstract |
In this study, we developed a procedure to produce gingivitis in rats by inoculation of Porphyromonas gingivalis and studied the contribution of the bacterial cysteine proteinases, Arg-gingipain (Rgp) and Lys-gingipain (Kgp), to the pathology in the gingiva. To adhere the bacterium to periodontal tissues, a cotton thread was inserted between the first and second molar of right maxillary sites of rats. Rats in group A were administered with vehicle alone after bacterial (strain W83) inoculation. In group B, the bacteria were inoculated in combination with leupeptin, a potent inhibitor of Rgp and Kgp, and then leupeptin alone was administered the week after. Rats in group C were administered leupeptin for 6 weeks after bacteria inoculation. All left maxillary gingiva in three groups showed no inflammatory changes. Right maxillary gingiva of group A showed most of the clinical landmarks of gingivitis. Leupeptin exhibited only a little inhibitory effect on this gingivitis in group B, whereas it had a strong inhibitory effect on the inflammation in group C. These results suggest that P. gingivalis-induced gingivitis is attributable to Rgp and Kgp and that leupeptin is more effective in the late phase than the early stage of gingivitis.
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Authors | S Kitano, K Irimura, T Sasaki, N Abe, A Baba, Y Miyake, N Katunuma, K Yamamoto |
Journal | Japanese journal of pharmacology
(Jpn J Pharmacol)
Vol. 85
Issue 1
Pg. 84-91
(Jan 2001)
ISSN: 0021-5198 [Print] Japan |
PMID | 11243579
(Publication Type: Journal Article)
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Chemical References |
- Adhesins, Bacterial
- Cysteine Proteinase Inhibitors
- Gingipain Cysteine Endopeptidases
- Hemagglutinins
- Leupeptins
- Cysteine Endopeptidases
- leupeptin
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Topics |
- Adhesins, Bacterial
(drug effects, metabolism)
- Animals
- Bacteroidaceae Infections
(drug therapy, microbiology)
- Cysteine Endopeptidases
(drug effects, metabolism)
- Cysteine Proteinase Inhibitors
(pharmacology, therapeutic use)
- Disease Models, Animal
- Disease Progression
- Gingipain Cysteine Endopeptidases
- Gingivitis
(drug therapy, microbiology, pathology)
- Hemagglutinins
(drug effects, metabolism)
- Leupeptins
(pharmacology, therapeutic use)
- Male
- Maxilla
(pathology)
- Porphyromonas gingivalis
(enzymology, pathogenicity)
- Rats
- Rats, Wistar
- Virulence
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