Comparison of the protective effect of melatonin with other antioxidants in the hamster kidney model of estradiol-induced DNA damage.

Abstract

17beta-Estradiol (E(2)) is a known carcinogen. Estrogen induction of tumors in hamster kidney is a model of estrogen-related carcinogenesis. Melatonin is a well-known antioxidant, free radical scavenger and oncostatic agent. Changes in the levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), an index of DNA damage, were measured in kidneys, liver and testes from hamsters treated with E(2) (75mg/kg b.w.) and collected 5h later. Potential protective effects of melatonin, N-acetylserotonin (NAS), indole-3-propionic acid (IPA) and ascorbic acid (AA) against E(2)-induced DNA damage were tested. The antioxidants were applied in equimolar doses of 64.5 micromol/kg b.w., 2 and 0.5h before and 2 and 4h after E(2) treatment. E(2) treatment caused a significant increase in 8-oxodGuo levels in kidneys, but did not influence significantly the oxidation of guanine bases in liver and testes. Melatonin, IPA and AA, but not NAS, completely prevented E(2)-induced DNA damage in hamster kidneys. It is concluded that melatonin, IPA and AA may be effective in protecting against E(2)-related DNA damage and, consequently, carcinogenesis.

• Authors: M Karbownik, R J Reiter, J Cabrera, J J Garcia
• Journal: Mutation research (Mutat Res) Vol. 474 Issue 1-2 Pg. 87-92 (Mar 01 2001) ISSN: 0027-5107 [Print] Netherlands
• PMID: 11239965 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Estradiol
• Melatonin

Topics

• Animals
• Antioxidants (pharmacology)
• Cricetinae
• DNA Damage
• Estradiol (toxicity)
• Kidney (drug effects, metabolism)
• Kidney Neoplasms (chemically induced, prevention & control)
• Melatonin (pharmacology)
• Mesocricetus