Abstract |
The rplK gene of Corynebacterium glutamicum ATCC13032 comprises 438 nucleotides and encodes a protein of 145 amino acids with a molecular mass of 15.3 kDa. The amino acid sequence revealed extensive similarities to the large ribosomal subunit protein L11 from several Gram-positive and Gram-negative bacteria. The C. glutamicum rplK gene is located downstream of secE, representing part of the protein export apparatus, and of nusG, encoding a transcription antiterminator protein. The rplK gene is followed by an ORF homologous to rplA encoding the 50S ribosomal protein L1. Northern analysis revealed that transcription of the rplK-rplA cluster resulted in two different transcripts of 1.5 and 0.6 kb. The 1.5 kb transcript corresponds to the entire rplK-rplA cluster and the short transcript originates from the rplK gene. A C. glutamicum rplK mutant strain carrying a 12 bp in-frame deletion within rplK, which resulted in the loss of the tetrapeptide Pro-Ala- Leu-Gly in the L11 protein, was constructed. The mutant failed to accumulate ( p)ppGpp in response to amino acid starvation and exhibited an increased tolerance to the antibiotic thiostrepton. Evidently, the C. glutamicum rplK gene is required for ( p)ppGpp accumulation upon nutritional starvation.
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Authors | Lutz Wehmeier, Olaf Brockmann-Gretza, Agustin Pisabarro, Andreas Tauch, Alfred Pühler, Juan F Martin, Jörn Kalinowski |
Journal | Microbiology (Reading, England)
(Microbiology (Reading))
Vol. 147
Issue Pt 3
Pg. 691-700
(Mar 2001)
ISSN: 1350-0872 [Print] England |
PMID | 11238976
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amino Acids
- Anti-Bacterial Agents
- Ribosomal Proteins
- ribosomal protein L11
- Guanosine Pentaphosphate
- Thiostrepton
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Topics |
- Amino Acid Sequence
- Amino Acids
(metabolism)
- Anti-Bacterial Agents
(pharmacology)
- Base Sequence
- Corynebacterium
(drug effects, genetics, physiology)
- Gene Deletion
- Guanosine Pentaphosphate
(metabolism)
- Molecular Sequence Data
- Ribosomal Proteins
(genetics, metabolism)
- Thiostrepton
(pharmacology)
- Transcription, Genetic
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