Abstract | OBJECTIVE: To investigate whether the development of osteoarthritic lesions in the knee joints of mice is associated with increased immunostaining of type X collagen. METHODS: Sections of total knee joints in combination with immunohistochemistry were used to study the distribution of type X collagen in the cartilage of young and old mice of two mouse strains, Balb/c and C57Bl/6, known to develop osteoarthritic lesions at different locations. Expression of type X collagen and PTH/PTHrP-receptor mRNA were studied by RT-PCR. RESULTS: Young adult Balb/c and C57Bl/6 mice both expressed type X collagen in the non-calcified cartilage of the tibia-femoral joint. Old mice of both strains had a strongly increased deposition of type X collagen in the patella-femoral but not in the tibia-femoral joint. The locations in the murine knee joints prone to develop osteoarthritis (OA) did not preferentially express increased amounts of type X collagen. Thus, whereas increased type X was observed in both strains in the patella-femoral joints, only Balb/c mice preferentially developed osteoarthritic lesions in these joints. Also cartilage degeneration was usually seen only in the lateral compartment of the knee joints of C57Bl/6 mice but this was not accompanied by increased type X collagen immunostaining. Increased deposition of type X collagen was not associated with elevated levels of type X collagen mRNA or with decreased levels of PTH/PTHrP-receptor mRNA. CONCLUSION:
Type X collagen expression and spontaneous OA in mice are not necessarily related since OA prone locations in the murine knee joint do not preferentially express type X collagen.
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Authors | P M van der Kraan, R Stoop, T H Meijers, A R Poole, W B van den Berg |
Journal | Osteoarthritis and cartilage
(Osteoarthritis Cartilage)
Vol. 9
Issue 2
Pg. 92-100
(Feb 2001)
ISSN: 1063-4584 [Print] England |
PMID | 11237656
(Publication Type: Journal Article)
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Copyright | Copyright 2001 |
Chemical References |
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Topics |
- Age Factors
- Animals
- Cartilage, Articular
(metabolism)
- Collagen
(chemistry)
- Hindlimb
(physiopathology)
- Joints
(physiopathology)
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Osteoarthritis, Knee
(physiopathology)
- Polymerase Chain Reaction
(methods)
- RNA, Messenger
(chemistry)
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