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Expression of type X collagen in young and old C57Bl/6 and Balb/c mice. Relation with articular cartilage degeneration.

AbstractOBJECTIVE:
To investigate whether the development of osteoarthritic lesions in the knee joints of mice is associated with increased immunostaining of type X collagen.
METHODS:
Sections of total knee joints in combination with immunohistochemistry were used to study the distribution of type X collagen in the cartilage of young and old mice of two mouse strains, Balb/c and C57Bl/6, known to develop osteoarthritic lesions at different locations. Expression of type X collagen and PTH/PTHrP-receptor mRNA were studied by RT-PCR.
RESULTS:
Young adult Balb/c and C57Bl/6 mice both expressed type X collagen in the non-calcified cartilage of the tibia-femoral joint. Old mice of both strains had a strongly increased deposition of type X collagen in the patella-femoral but not in the tibia-femoral joint. The locations in the murine knee joints prone to develop osteoarthritis (OA) did not preferentially express increased amounts of type X collagen. Thus, whereas increased type X was observed in both strains in the patella-femoral joints, only Balb/c mice preferentially developed osteoarthritic lesions in these joints. Also cartilage degeneration was usually seen only in the lateral compartment of the knee joints of C57Bl/6 mice but this was not accompanied by increased type X collagen immunostaining. Increased deposition of type X collagen was not associated with elevated levels of type X collagen mRNA or with decreased levels of PTH/PTHrP-receptor mRNA.
CONCLUSION:
Type X collagen expression and spontaneous OA in mice are not necessarily related since OA prone locations in the murine knee joint do not preferentially express type X collagen.
AuthorsP M van der Kraan, R Stoop, T H Meijers, A R Poole, W B van den Berg
JournalOsteoarthritis and cartilage (Osteoarthritis Cartilage) Vol. 9 Issue 2 Pg. 92-100 (Feb 2001) ISSN: 1063-4584 [Print] England
PMID11237656 (Publication Type: Journal Article)
CopyrightCopyright 2001
Chemical References
  • RNA, Messenger
  • Collagen
Topics
  • Age Factors
  • Animals
  • Cartilage, Articular (metabolism)
  • Collagen (chemistry)
  • Hindlimb (physiopathology)
  • Joints (physiopathology)
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Osteoarthritis, Knee (physiopathology)
  • Polymerase Chain Reaction (methods)
  • RNA, Messenger (chemistry)

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