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Alterations in osteoclast morphology following long-term 17beta-estradiol administration in the mouse.

AbstractBACKGROUND:
Although the role of the osteoclast in bone resorption is becoming better understood, much remains to be learned about osteoclastogenesis and the exact mechanism of action of anti-resorbing agents such as 17beta-estradiol. This study investigated bone and morphologic osteoclast alterations following long-term estrogen administration to the B6D2F1 mouse. B6D2F1 mice aged 4-5 weeks were exposed to high levels of estrogen via implanted silastic tubing for at least 12 weeks; controls received empty tubing. Femurs of control and treated mice were assessed with radiology, quantitative histomorphometry and transmission electron microscopy.
RESULTS:
After 8 weeks of treatment, there was radiologic evidence of severe osteosclerosis and 86% of femoral marrow space was replaced with bone. After 12 weeks histologic studies of treated animals revealed that osteoclasts were positive for tartrate-resistant acid phosphatase but showed markedly abnormal ultrastructure which prevented successful bone resorption.
CONCLUSIONS:
Findings extend our understanding of osteoclast structure and function in the mouse exposed in vivo to high doses of estrogen. Ultrastructural examination showed that osteoclasts from estrogen-treated mice were unable to seal against the bone surface and were unable to form ruffled borders.
AuthorsH E Gruber, I J Puzanov, M Bennett, V Kumar, B Gordon
JournalBMC cell biology (BMC Cell Biol) Vol. 2 Pg. 3 ( 2001) ISSN: 1471-2121 [Electronic] England
PMID11231877 (Publication Type: Journal Article)
Chemical References
  • Isoenzymes
  • Estradiol
  • Acid Phosphatase
  • Tartrate-Resistant Acid Phosphatase
Topics
  • Acid Phosphatase (analysis)
  • Animals
  • Estradiol (pharmacology)
  • Femur (anatomy & histology, diagnostic imaging, ultrastructure)
  • Isoenzymes (analysis)
  • Kinetics
  • Mice
  • Osteoclasts (drug effects, enzymology, ultrastructure)
  • Radiography
  • Tartrate-Resistant Acid Phosphatase

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