Redox control of Epstein-Barr virus replication by human thioredoxin/ATL-derived factor: differential regulation of lytic and latent infection.

Abstract

Human thioredoxin (hTRX)/adult T-cell leukemia (ATL)-derived factor (ADF) was originally reported as an interleukin-2 (IL-2) receptor-alpha-inducing factor produced by human T-cell lymphotropic virus-1-positive (HTLV-1+) cell lines. Growing evidence indicates that hTRX/ADF plays important roles in cellular responses against oxidative stress and is involved in a variety of cellular functions. A high level of hTRX/ADF expression is also observed in other human virus-infected cell lines including those of Epstein-Barr virus (EBV) and human papillomavirus. In this report, we analyzed the effect of hTRX/ADF on lytic amplification and persistent replication of EBV as a model for lytic versus latent phase of viral replication in host cells. Addition of hTRX/ADF clearly suppressed lytic replication of EBV in Raji cells and B95-8 cells induced to the lytic phase of 12-O-tetradecanoylphorbol-13-acetate (TPA), and it prevented the death of these cells evoked by the lytic induction. In contrast, hTRX/ADF did not have any effect on persistent replication in the latent phase. These data indicated that hTRX/ADF prevents EBV-transformed cells from proceeding into the lytic phase and regulates cohabitation of EBV and its host cells.

• Authors: H Sono, K Teshigawara, T Sasada, Y Takagi, A Nishiyama, Y Ohkubo, Y Maeda, E Tatsumi, A Kanamaru, J Yodoi
• Journal: Antioxidants & redox signaling (Antioxid Redox Signal) Vol. 1 Issue 2 Pg. 155-65 ( 1999) ISSN: 1523-0864 [Print] United States
• PMID: 11228744 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinogens
• Cytokines
• DNA, Viral
• Neoplasm Proteins
• adult T cell leukemia-derived factor
• Thioredoxins
• Tetradecanoylphorbol Acetate

Topics

• Carcinogens (pharmacology)
• Cell Death (drug effects)
• Cell Line, Transformed (virology)
• Cell Transformation, Viral (genetics)
• Cytokines (genetics, metabolism, physiology)
• DNA Replication (genetics)
• DNA, Viral (antagonists & inhibitors, genetics)
• Gene Amplification
• Gene Expression Regulation, Viral
• Herpesvirus 4, Human (genetics, metabolism, pathogenicity, physiology)
• Humans
• Infectious Mononucleosis (genetics, metabolism, virology)
• Leukemia-Lymphoma, Adult T-Cell (virology)
• Neoplasm Proteins (genetics, metabolism, physiology)
• Oxidation-Reduction
• Suppression, Genetic
• Tetradecanoylphorbol Acetate (pharmacology)
• Thioredoxins (pharmacology)
• Virus Replication (genetics)