Inhibition of caspase-1 induces cell death in pancreatic carcinoma cells and potentially modulates expression levels of bcl-2 family proteins.

Abstract

Caspase-1 (interleukin-1beta-converting enzyme) is reported to play an important role in the regulation of apoptosis. We investigated the inhibition of caspase-1 by the cell permeable caspase-1 inhibitor Ac-AAVALLPAVLLALLAP-YVAD.CHO in pancreatic carcinoma cells. Inhibition of caspase-1 induced a non-apoptotic/"necrotic-like" cell death in AsPC-1, BxPC-3, MiaPaCa-2 and Panc-1 cells. Expression levels of bcl-2 and bax were up-regulated in caspase-1 inhibitor-treated cells while that of bcl-x(L) remained unaltered. Our observations support our previous findings that caspase-1 is potentially involved in anti-apoptotic processes in pancreatic carcinoma.

• Authors: S Schlosser, F Gansauge, M Ramadani, H G Beger, S Gansauge
• Journal: FEBS letters (FEBS Lett) Vol. 491 Issue 1-2 Pg. 104-8 (Feb 23 2001) ISSN: 0014-5793 [Print] England
• PMID: 11226429 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ac-AAVALLPAVLLALLAPYVAD-CHO
• BAX protein, human
• Caspase Inhibitors
• Oligopeptides
• Proto-Oncogene Proteins
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• Caspase 1

Topics

• Apoptosis
• Blotting, Western
• Caspase 1 (metabolism)
• Caspase Inhibitors
• Cell Cycle
• DNA Fragmentation
• Humans
• Necrosis
• Oligopeptides (pharmacology)
• Pancreatic Neoplasms
• Proto-Oncogene Proteins (metabolism)
• Proto-Oncogene Proteins c-bcl-2 (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured
• Up-Regulation
• bcl-2-Associated X Protein