In order to determine key
MMPs for invasion and
metastasis in various human
cancers, we examined the expression of ten
MMPs (
MMP-1, 2, 3, 7, 8, 9, 13 and MT1, 2, 3-
MMPs) and tissue inhibitors of
metalloproteinases (TIMP-1 and 2) in
breast carcinomas, thyroid
papillary carcinomas,
endometrial carcinomas, ovarian
carcinomas, gastric
adenocarcinomas,
oral squamous cell carcinomas and
gliomas. Of the
MMPs examined, the activation of
proMMP-2 by
MT1-MMP (membrane type 1-
MMP) was commonly important for the invasion and
metastasis of these
cancers except for
endometrial carcinomas. The MMP-2 and
MT1-MMP were localized to the
carcinoma cells and gelatinolytic activity was demonstrated within the
carcinoma cell nests by in situ zymography. In
endometrial carcinomas, production and activation of
proMMP-7 were a key determinant of the
lymph node metastasis. The activation of
proMMP-2 in
gliomas involved
MT2-MMP as well as
MT1-MMP, and a combination of decreased
TIMP-2 production and enhanced
MT1-MMP expression was important in the subarachnoidal dissemination of
glioblastoma cells.
Brevican, a major adult brain
proteoglycan, was degraded with MMP-1, 2, 3, 7, 10 and ADAMTS4 (aggrecanase-1) by being cleaved at the
MMP site (the Ala360-Phe361 bond) with the
MMPs and ADAM site (the Glu395-Ser396 bond) with ADAMTS4. Since activated MMP-2 and ADAMTS4 are present in human
glioma tissues, they may play a key role in the invasion of
glioma cells through the
brevican degradation. The data in the present study suggest that the extracellular matrix-degrading
metalloproteinases acting probably on the cell membranes of
cancer cells are essential to the invasion and
metastasis of human
cancers.