Abstract |
We investigated the susceptibility of three inbred strains of rats to the hepatocarcinogen, N-nitrosomorpholine (NNM), to establish a spontaneous metastatic model of hepatocellular carcinoma (HCC). WS/Shi. SD/gShi, and F344/DuCrj rats were given 0.02% NNM in drinking water for 8 weeks and thereafter left without any treatment. The experiment ceased at week 20, because mortality markedly increased after this time point in WS/Shi rats. Liver weight was highest in WS/Shi rats among the three strains examined. The incidence of HCC was 15/15 (100%) in WS/Shi rats, 1/16 (6%) in SD/gShi rats, and 13/16 (81%) in F344/DuCrj rats surviving after NNM treatment. Metastasis to the lung was observed in HCC-bearing rats at an incidence of 13/15 (87%) in WS/Shi, 1/1 in SD/gShi, and 6/13 (46%) in F344/DuCrj. Four-week administration of NNM resulted in a significantly higher BrdU-labeling index of hepatocytes in WS/Shi rats than in the other strains. These findings indicated that WS/Shi is the most sensitive strain to NNM and may be the most suitable strain for use as a spontaneous metastatic model of HCC among the strains of rats examined in the present study.
|
Authors | T Murai, S Mori, M Hosono, Y Iwakura, A Takashima, T Oohara, S Makino, R Takeda, S Fukushima |
Journal | Oncology research
(Oncol Res)
Vol. 12
Issue 3
Pg. 121-6
( 2000)
ISSN: 0965-0407 [Print] United States |
PMID | 11216670
(Publication Type: Journal Article)
|
Chemical References |
- Carcinogens
- Nitrosamines
- N-nitrosomorpholine
|
Topics |
- Animals
- Carcinogens
(toxicity)
- Disease Susceptibility
- Liver Neoplasms, Experimental
(chemically induced, pathology)
- Lung Neoplasms
(secondary)
- Male
- Nitrosamines
(toxicity)
- Rats
- Rats, Inbred F344
- Rats, Inbred Strains
- Rats, Sprague-Dawley
- Rats, Wistar
- Species Specificity
|