Abstract | BACKGROUND AND AIMS: METHODS AND RESULTS: The administration of LPS (5 mg/kg) intraperitoneally resulted in a dramatic upregulation of PAP-I mRNA expression, increasing 18.61-fold to a maximum at 12 h, then decreasing, but still sustaining at a high level and reaching baseline on day five. These changes were accompanied by the upregulation of tumor necrosis factor ( TNF)-alpha, interleukin-1beta (IL-1beta), interleukin 6 (IL-6) and interferon gamma (IFNgamma) mRNA expressions in the pancreas, but not by marked alterations of serum amylase, lactic dehydrogenase (LDH) and histology. Cerulein also increased PAP-I mRNA expression. However, the combination of cerulein and LPS was not able to enhance PAP-I mRNA expression further, although more prominent pancreatitis based on significant changes of serum amylase, LDH and histology were observed. CONCLUSION:
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Authors | X Wang, B Wang, J Wu |
Journal | Journal of gastroenterology and hepatology
(J Gastroenterol Hepatol)
Vol. 16
Issue 1
Pg. 79-86
(Jan 2001)
ISSN: 0815-9319 [Print] Australia |
PMID | 11206320
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Acute-Phase Proteins
- Antigens, Neoplasm
- Biomarkers, Tumor
- Cytokines
- Lectins, C-Type
- Lipopolysaccharides
- Pancreatitis-Associated Proteins
- Proteins
- REG3A protein, human
- RNA, Messenger
- Reg3b protein, mouse
- Ceruletide
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Topics |
- Acute Disease
- Acute-Phase Proteins
(metabolism)
- Animals
- Antigens, Neoplasm
- Biomarkers, Tumor
- Blotting, Northern
- Ceruletide
- Cytokines
(metabolism)
- Immunohistochemistry
- Lectins, C-Type
- Lipopolysaccharides
(pharmacology)
- Mice
- Mice, Inbred C57BL
- Pancreatitis
(chemically induced, metabolism)
- Pancreatitis-Associated Proteins
- Proteins
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Up-Regulation
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