HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Altered response to thyroid hormones by breast and ovarian cancer cells.

AbstractBACKGROUND:
In this study, L-thyroxine (T4), 3',3,5-triiodo-L-thyronine (T3), 3,5-diiodo-L-thyronine (T2), reverse T3; 3',5',3-triiodo-L-thyronine (RT3) and transferrin were added to breast cancer cell lines Hs 578T, MDA-MB-231, MDA-MB-468, and T-47D and ovarian cancer cell line OVCAR-3 to test the response to cell proliferation.
MATERIALS AND METHODS:
Breast and ovarian cancer cell lines were placed in serum-free medium prior to addition of effector. Proliferation was determined by thymidine incorporation. For Northern analysis, RNA was isolated and c-fos, cjun and TIEG expression assessed.
RESULTS:
No compound provided uniform results across all cell lines. T2 inhibited proliferation in Hs 578T and MDA-MB-468, had no effect in MDA-MB-231 and OVCAR-3, and stimulated proliferation in T-47D cells. T3 inhibited proliferation in all cell lines except T-47D in which two-state behavior occurred, with increased proliferation at low concentrations (< or = 10(-6) M) and decreased proliferation at high concentrations (> or = 10(-5) M). RT3 inhibited proliferation in Hs 578T, MDA-MB-231, and T-47D but had no effect in MDA-MB-468 and OVCAR-3. T4 inhibited proliferation in Hs 578T, MDA-MB-231, and MDA-MB-468 and had two-state behavior in T-47D and OVCAR-3. Finally, transferrin increased proliferation only in OVCAR-3 cells. Protooncogene expression was increased by both transferrin and T4 in the cell lines tested.
CONCLUSIONS:
Correlation of iodines and proliferative responses were used to determine "essential" iodines necessary to produce the observed effect. Interaction between these cancer cells and non-physiological concentrations of thyroid hormone can be explained by thyroid hormone receptors with altered binding properties. Thus, interaction of thyroid hormones and cancer cells may differ from what occurs with normal cells.
AuthorsM B Martinez, M Ruan, L A Fitzpatrick
JournalAnticancer research (Anticancer Res) 2000 Nov-Dec Vol. 20 Issue 6B Pg. 4141-6 ISSN: 0250-7005 [Print] Greece
PMID11205239 (Publication Type: Journal Article)
Chemical References
  • Diiodothyronines
  • Proto-Oncogene Proteins c-fos
  • Thyroid Hormones
  • Transferrin
  • Triiodothyronine
  • 3,5-diiodothyronine
  • Triiodothyronine, Reverse
  • Thyroxine
Topics
  • Breast Neoplasms (pathology)
  • Cell Division (drug effects)
  • Diiodothyronines (pharmacology)
  • Female
  • Humans
  • Male
  • Prostatic Neoplasms (pathology)
  • Proto-Oncogene Proteins c-fos (pharmacology)
  • Thyroid Hormones (pharmacology)
  • Thyroxine (pharmacology)
  • Transferrin (pharmacology)
  • Triiodothyronine (pharmacology)
  • Triiodothyronine, Reverse (pharmacology)
  • Tumor Cells, Cultured (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: