Quantitative determination of L-775,606, a selective 5-hydroxytryptamine 1D agonist, in rat plasma using automated sample preparation and detection by liquid chromatography-tandem mass spectrometry.

Abstract

L-775,606 is under investigation as a selective 5-hydroxytryptamine 1D agonist for the treatment of migraine. A reliable and sensitive method for the analysis of L-775,606 in plasma was required in order to support preclinical evaluation of this compound. A semi-automated sample preparation method using the Beckman Biomek 2000 workstation to perform all liquid handling tasks has been established. The sample analysis was performed using HPLC-MS-MS with a cycle time of 3.5 min per sample. Intra- and inter-day assay accuracy and precision are excellent with a calibration range of 1-2000 ng/ml and a reproducible limit of quantification of 1 ng/ml.

• Authors: K L Locker, D Morrison, A P Watt
• Journal: Journal of chromatography. B, Biomedical sciences and applications (J Chromatogr B Biomed Sci Appl) Vol. 750 Issue 1 Pg. 13-23 (Jan 05 2001) ISSN: 1387-2273 [Print] Netherlands
• PMID: 11204213 (Publication Type: Journal Article)

Chemical References

• 1-(3-(5-(1,2,4-triazol-4-yl)-1H-indol-3-yl)propyl)-4-(2-(3-fluorophenyl)ethyl)piperazine
• Indoles
• Piperazines
• Receptor, Serotonin, 5-HT1D
• Receptors, Serotonin
• Serotonin Receptor Agonists

Topics

• Animals
• Automation
• Calibration
• Chromatography, Liquid (methods)
• Indoles (blood)
• Male
• Mass Spectrometry (methods)
• Piperazines (blood)
• Rats
• Rats, Sprague-Dawley
• Receptor, Serotonin, 5-HT1D
• Receptors, Serotonin (drug effects)
• Reproducibility of Results
• Sensitivity and Specificity
• Serotonin Receptor Agonists (blood)