Two cases of severe bronchopneumonia due to influenza A (H3N2) virus: detection of influenza virus gene using reverse transcription polymerase chain reaction.

Abstract	We report two cases of severe bronchopneumonia due to influenza A (H3N2) virus. The severity of the disease necessitated initiation of empiric therapy based on the present illness and clinical data on admission. Both patients were improved by artificial ventilation with positive end-expiratory pressures and administration of broad spectrum antibiotics and corticosteroids before confirming the diagnosis of viral bronchopneumonia using viral culture and serological tests. Within 24 hours, influenza A (H3N2) virus was identified by amplification of the pathogen genes by reverse transcription polymerase chain reaction (RT-PCR) using the stored bronchoalveolar lavage (BAL) fluids of both cases. This suggests that a combination of detection methods of pathogens using RT-PCR and BAL fluid will facilitate determination of rational treatment aimed at influenza A virus.
Authors	M Doi, S Takao, K Kaneko, S Karakawa, S Ishihara, Y Awaya, M Kuwabara, S Ishioka, M Yamakido
Journal	Internal medicine (Tokyo, Japan) (Intern Med) Vol. 40 Issue 1 Pg. 61-7 (Jan 2001) ISSN: 0918-2918 [Print] Japan
PMID	11201374 (Publication Type: Case Reports, Journal Article)
Chemical References	Cephalosporins RNA, Viral Fosfomycin Clindamycin Betamethasone Minocycline flomoxef Methylprednisolone
Topics	Aged Antibiotic Prophylaxis Betamethasone (therapeutic use) Bronchoalveolar Lavage Fluid (virology) Bronchopneumonia (etiology, therapy, virology) Cephalosporins (therapeutic use) Clindamycin (therapeutic use) Combined Modality Therapy Drug Therapy, Combination (therapeutic use) Fosfomycin (therapeutic use) Humans Influenza A Virus, H3N2 Subtype Influenza A virus (genetics, isolation & purification) Male Methylprednisolone (therapeutic use) Middle Aged Minocycline (therapeutic use) Pneumonia, Viral (etiology, therapy, virology) Positive-Pressure Respiration RNA, Viral (analysis) Respiration, Artificial Reverse Transcriptase Polymerase Chain Reaction

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