HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Antisense phosphorothioate oligonucleotides targeted to the human chemokine receptor CXCR4.

Abstract
The CXC chemokine receptor CXCR4 is used as a major co-receptor for fusion and entry by syncytia-inducing T-tropic (X4) isolates of HIV-1. In the present study, we report the effects of an antisense oligodeoxyribonucleotide on the inhibition of CXCR4 gene expression in X4 HIV-1 infected HeLa-CD4 cells, to find more efficacious therapeutic possibilities for Human Immunodeficiency Virus type 1 (HIV-1) infection. Antisense phosphorothioate oligodeoxyribonucleotides (anti-S-ODNs) corresponding to the sequence of bases 69 to 88 of the human CXCR4 mRNA gene were synthesized. When the naked anti-S-ODN was incubated with HeLa-CD4 cells, the surface levels of this chemokine receptor were reduced up to 50%, indicating sequence-specific inhibition. We also examined the concomitant use of a basic peptide transfection reagent, nucleosomal histone proteins (RNP), for delivery of anti-S-ODNs. The anti-S-ODN encapsulated with RNP had higher inhibitory effects on p24 products than the naked anti-S-ODN.
AuthorsA Kusunoki, N Miyano-Kurosaki, T Kimura, K Takai, N Yamamoto, H Gushima, H Takaku
JournalNucleosides, nucleotides & nucleic acids (Nucleosides Nucleotides Nucleic Acids) 2000 Oct-Dec Vol. 19 Issue 10-12 Pg. 1709-19 ISSN: 1525-7770 [Print] United States
PMID11200267 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Oligonucleotides, Antisense
  • Organophosphorus Compounds
  • Receptors, CXCR4
  • Thionucleotides
Topics
  • Base Sequence
  • HeLa Cells
  • Humans
  • Oligonucleotides, Antisense (chemistry, pharmacology)
  • Organophosphorus Compounds (chemistry)
  • Receptors, CXCR4 (drug effects)
  • Thionucleotides (chemistry, pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: