Abstract |
The CXC chemokine receptor CXCR4 is used as a major co-receptor for fusion and entry by syncytia-inducing T-tropic (X4) isolates of HIV-1. In the present study, we report the effects of an antisense oligodeoxyribonucleotide on the inhibition of CXCR4 gene expression in X4 HIV-1 infected HeLa-CD4 cells, to find more efficacious therapeutic possibilities for Human Immunodeficiency Virus type 1 (HIV-1) infection. Antisense phosphorothioate oligodeoxyribonucleotides (anti-S-ODNs) corresponding to the sequence of bases 69 to 88 of the human CXCR4 mRNA gene were synthesized. When the naked anti-S-ODN was incubated with HeLa-CD4 cells, the surface levels of this chemokine receptor were reduced up to 50%, indicating sequence-specific inhibition. We also examined the concomitant use of a basic peptide transfection reagent, nucleosomal histone proteins (RNP), for delivery of anti-S-ODNs. The anti-S-ODN encapsulated with RNP had higher inhibitory effects on p24 products than the naked anti-S-ODN.
|
Authors | A Kusunoki, N Miyano-Kurosaki, T Kimura, K Takai, N Yamamoto, H Gushima, H Takaku |
Journal | Nucleosides, nucleotides & nucleic acids
(Nucleosides Nucleotides Nucleic Acids)
2000 Oct-Dec
Vol. 19
Issue 10-12
Pg. 1709-19
ISSN: 1525-7770 [Print] United States |
PMID | 11200267
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Oligonucleotides, Antisense
- Organophosphorus Compounds
- Receptors, CXCR4
- Thionucleotides
|
Topics |
- Base Sequence
- HeLa Cells
- Humans
- Oligonucleotides, Antisense
(chemistry, pharmacology)
- Organophosphorus Compounds
(chemistry)
- Receptors, CXCR4
(drug effects)
- Thionucleotides
(chemistry, pharmacology)
|