Abstract |
We investigated whether IL-10 produced endogenously would influence the development of HSV-1-induced acute corneal disease. Murine corneal epithelial cells and fibroblasts cultured in vitro expressed IL-10 mRNA and protein constitutively and also IL-10 receptors. Inclusion of IL-10 neutralizing antibody in the culture medium significantly (p<0.05) enhanced TNF-alpha-induced IL-6 and MIP-2 production by both corneal cell types. Endogenous IL-10 synthesis, which also occurred in vivo, was not modulated by Herpes virus infection or by depletion of neutrophils or natural killer cells. Antibody to IL-10 given locally at the time of HSV-1 intracorneal infection was associated with significantly (p<0.05) enhanced production of IL-6, MIP-2, and MIP-1alpha, increased neutrophil infiltration, and more extensive corneal disease. Similarly, mice with a disrupted IL-10 gene developed more severe corneal disease than wild-type controls. Collectively, these observations suggest that locally produced IL-10 can act in an autocrine/paracrine fashion to down-regulate the production of proinflammatory mediators and thus limit corneal inflammation.
|
Authors | X T Yan, M Zhuang, J E Oakes, R N Lausch |
Journal | Journal of leukocyte biology
(J Leukoc Biol)
Vol. 69
Issue 1
Pg. 149-57
(Jan 2001)
ISSN: 0741-5400 [Print] United States |
PMID | 11200059
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
|
Topics |
- Animals
- Autocrine Communication
(immunology)
- Cornea
(immunology, virology)
- Corneal Diseases
(immunology, virology)
- Female
- Herpes Simplex
(immunology)
- Herpesvirus 1, Human
(immunology)
- Inflammation
(immunology)
- Interleukin-10
(immunology)
- Mice
- Mice, Inbred BALB C
- Mice, Knockout
|