Abstract | UNLABELLED: Recent clinical data indicate that tumor hypoxia negatively affects the treatment outcome of both radiotherapy and surgery in various cancers, emphasizing the need for noninvasive detection of tumor hypoxia. Several studies have shown an increased uptake of FDG in hypoxic regions of xenografts, suggesting the use of PET with FDG as a potential technique. In this study, we examine the mechanism underlying the hypoxia-induced increase of FDG uptake in the human breast carcinoma cell line MCF7. METHODS: The uptake of 3H-FDG into MCF7 cells was determined after incubation under hypoxic (0% oxygen) or normoxic conditions, with or without redox agents, for varying time periods. In addition, the effects of the redox agents on the glucose transporter activity and the hexokinase activity were determined independently, and the effects of hypoxia on glucose transporter protein and hexokinase levels were assessed. RESULTS: CONCLUSION:
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Authors | P Burgman, J A Odonoghue, J L Humm, C C Ling |
Journal | Journal of nuclear medicine : official publication, Society of Nuclear Medicine
(J Nucl Med)
Vol. 42
Issue 1
Pg. 170-5
(Jan 2001)
ISSN: 0161-5505 [Print] United States |
PMID | 11197971
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Monosaccharide Transport Proteins
- Fluorodeoxyglucose F18
- Tritium
- 4-Chloromercuribenzenesulfonate
- Hexokinase
- Dithiothreitol
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Topics |
- 4-Chloromercuribenzenesulfonate
(pharmacology)
- Breast Neoplasms
(metabolism)
- Cell Hypoxia
- Cell Line
- Dithiothreitol
(pharmacology)
- Female
- Fluorodeoxyglucose F18
(pharmacokinetics)
- Hexokinase
(metabolism)
- Humans
- Monosaccharide Transport Proteins
(metabolism)
- Tritium
- Tumor Cells, Cultured
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