Abstract | PURPOSE: METHODS: A standardized 5.5-mm diameter penetrating central corneal alkali wound was induced in one eye in each of ten New Zealand white rabbits (2.5 kg). In five of the ten rabbits, 1.5 mL 5% fucoidin was given intravenously every 2 hours to prevent leukocytes from leaving the blood stream. Presence of hyaluronan (HA) and proliferating cell nuclear antigen ( PCNA) in the corneas were analyzed using immunohistochemical staining 36 hours after injury. RESULTS: In the alkali wounded corneas, HA was expressed intensively in the limbal area where a massive infiltration of leukocytes was seen. PCNA was expressed in the vascular endothelium as well as in the corneal cells. In the leukocyte-free corneas, HA staining intensity and distribution were the same as in uninjured corneas. No positive PCNA staining was seen in the vascular endothelial cells in these corneas. CONCLUSIONS: Extravasated leukocytes in the alkali-burned corneas caused enhanced production of HA and proliferation of vascular endothelial cells.
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Authors | L Gan, P Fagerholm |
Journal | Cornea
(Cornea)
Vol. 20
Issue 1
Pg. 96-9
(Jan 2001)
ISSN: 0277-3740 [Print] United States |
PMID | 11189012
(Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Alkalies
- Biomarkers
- Proliferating Cell Nuclear Antigen
- Hyaluronic Acid
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Topics |
- Alkalies
(toxicity)
- Animals
- Biomarkers
- Burns, Chemical
(complications, pathology)
- Cornea
(metabolism)
- Corneal Injuries
- Corneal Neovascularization
(etiology, metabolism, pathology)
- Endothelium, Vascular
(metabolism, pathology)
- Eye Burns
(complications, pathology)
- Hyaluronic Acid
(metabolism)
- Leukocytes
(metabolism, pathology)
- Proliferating Cell Nuclear Antigen
(metabolism)
- Rabbits
- Wound Healing
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