Comparative structure, proximal promoter elements, and chromosome location of the human eosinophil major basic protein genes.

Abstract	Human eosinophil major basic protein (MBP) is strongly implicated as a mediator of disease, especially bronchial asthma. We recently isolated a highly divergent human homologue of MBP (MBPH). Given human MBP's importance in disease and the restricted expression of it and human MBPH, we isolated the 4.6-kb human MBPH gene (HGMW-approved symbol PRG3). Comparisons among the human MBP (PRG2), human MBPH, and murine MBP-1 (mMBP-1; Prg2) genes suggest that the human MBP and mMBP-1 genes are more closely related than either is to the human MBPH gene. Proximal promoters of these three genes show conservation of potential binding sites for IK2 and STAT and of a known GATA site. However, a known C/EBP site is altered in the human MBPH gene's proximal promoter. The human MBP and MBPH genes localized to chromosome 11 in the centromere to 11q12 region. Thus, the human MBP and MBPH genes have diverged considerably, probably following a gene duplication event. Furthermore, the identified conserved and distinct proximal promoter elements likely contribute to the eosinophil-restricted and relatively reduced transcription of the human MBPH gene.
Authors	D A Plager, D A Weiler, D A Loegering, W B Johnson, L Haley, R L Eddy, T B Shows, G J Gleich
Journal	Genomics (Genomics) Vol. 71 Issue 3 Pg. 271-81 (Feb 01 2001) ISSN: 0888-7543 [Print] United States
PMID	11170744 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Copyright	Copyright 2001 Academic Press.
Chemical References	Blood Proteins CCAAT-Enhancer-Binding Proteins DNA, Complementary Eosinophil Granule Proteins Prg3 protein, mouse Ribonucleases Eosinophil Major Basic Protein
Topics	Animals Base Sequence Binding Sites Blood Proteins (biosynthesis, genetics) CCAAT-Enhancer-Binding Proteins (metabolism) Centromere (ultrastructure) Chromosome Mapping Chromosomes, Human, Pair 11 Conserved Sequence DNA, Complementary (metabolism) Eosinophil Granule Proteins Eosinophil Major Basic Protein Evolution, Molecular Exons Gene Library Humans In Situ Hybridization, Fluorescence Introns Mice Models, Genetic Molecular Sequence Data Promoter Regions, Genetic Ribonucleases Sequence Analysis, DNA Sequence Homology, Nucleic Acid Transcription, Genetic

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