HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Effects of interleukin-10 (IL-10) on pain behavior and gene expression following excitotoxic spinal cord injury in the rat.

Abstract
Intraspinal injection of quisqualic acid (QUIS) produces excitotoxic injury with pathophysiological characteristics similar to those associated with ischemic and traumatic spinal cord injury (SCI). Responses to QUIS-induced injury include an inflammatory component, as well as the development of spontaneous and evoked pain behaviors. We hypothesized that QUIS-induced inflammation and subsequent gene expression contribute to the development and progression of pain-related behaviors and that blockade of inflammation-related gene expression leads to the amelioration of these behaviors. Using the QUIS model of spinal cord injury, we examined whether interleukin-10 (IL-10), a potent anti-inflammatory cytokine, is able to reduce mRNA levels of inflammatory and cell death-related genes leading to a reduction of pain behaviors. The results demonstrate that animals receiving systemic injection of IL-10, 30 minutes following QUIS-induced SCI, showed a significant delay in the onset of excessive grooming behavior, a significant reduction in grooming severity, and a significant reduction in the longitudinal extent of a pattern of neuronal loss within the spinal cord characterized as "grooming-type damage." QUIS injections also resulted in an increase in mRNA levels of interleukin-1 beta (IL-1 beta), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), CD95 ligand (CD95-L, also called FAS-L/APO-1L), and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Results of QUIS injury plus IL-10 treatment resulted in a significant downregulation of IL1-beta and iNOS mRNA and these results were supported by Western blot analysis of protein levels following IL-10 treatment. These data suggest that IL-10 reduces inflammation and that targeting injury-induced inflammation is an effective strategy for limiting the extent of neuronal damage following excitotoxic SCI and thus the onset and progression of injury-induced pain behaviors.
AuthorsJ A Plunkett, C G Yu, J M Easton, J R Bethea, R P Yezierski
JournalExperimental neurology (Exp Neurol) Vol. 168 Issue 1 Pg. 144-54 (Mar 2001) ISSN: 0014-4886 [Print] United States
PMID11170729 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
CopyrightCopyright 2001 Academic Press.
Chemical References
  • Apoptosis Regulatory Proteins
  • FASLG protein, human
  • Fas Ligand Protein
  • Faslg protein, rat
  • Isoenzymes
  • Membrane Glycoproteins
  • Membrane Proteins
  • TNF-Related Apoptosis-Inducing Ligand
  • TNFSF10 protein, human
  • Tnfsf10 protein, rat
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Quisqualic Acid
  • NOS2 protein, human
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II
  • Nos2 protein, rat
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases
Topics
  • Animals
  • Apoptosis Regulatory Proteins
  • Cyclooxygenase 2
  • Fas Ligand Protein
  • Gene Expression Regulation (drug effects, physiology)
  • Grooming (drug effects)
  • Humans
  • Injections, Spinal
  • Interleukin-10 (administration & dosage, genetics, pharmacology)
  • Isoenzymes (genetics)
  • Male
  • Membrane Glycoproteins (genetics)
  • Membrane Proteins
  • Nitric Oxide Synthase (genetics)
  • Nitric Oxide Synthase Type II
  • Pain (physiopathology)
  • Pia Mater
  • Prostaglandin-Endoperoxide Synthases (genetics)
  • Quisqualic Acid (administration & dosage, toxicity)
  • Rats
  • Rats, Sprague-Dawley
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin (drug effects, pathology)
  • Spinal Cord (drug effects, metabolism, physiopathology)
  • Spinal Cord Injuries (chemically induced, physiopathology)
  • TNF-Related Apoptosis-Inducing Ligand
  • Time Factors
  • Transcription, Genetic (drug effects)
  • Tumor Necrosis Factor-alpha (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: