Abstract | BACKGROUND: Intravenous anaesthetics and sedatives can influence polymorphonuclear cell (PMN) functions. Some of the drugs for sedation and anaesthesia have been alternatively dissolved in lipid solutions containing medium (MCT) and/or long chain (LCT) triglycerides. The in vitro effects of two different diazepam ( benzyl-alcohol, LCT/MCT), etomidate ( propylene-glycol, LCT/MCT), and propofol (LCT, LCT/MCT) preparations on respiratory burst (RB) and phagocytosis of human PMNs were studied. METHODS:
Diazepam (2, 20 microg ml(-1)), etomidate (0.5, 5 microg ml(-1)), and propofol (6, 60 microg ml(-1)) were investigated in clinical and 10-fold concentrations with flow cytometric assays. The RB was measured with the fluorescent dye rhodamine after induction with Escherichia coli or formyl-methionyl- leucylphenylalanine (FMLP) following priming with tumour necrosis factor alpha ( TNF-alpha). Phagocytosis of PMNs was carried out in whole blood after incubation with fluorescein-labelled E. coli. RESULTS: LCT- propofol at 60 microg ml(-1) reduced the percentage of PMNs with RB activity after induction with E. coli (52.8+/-20.4) and TNF-alpha/FMLP (10.8+/-5.1)) as well as the percentage of phagocytosing PMNs (48.9+/-19.5) in contrast to LCT/MCT- propofol, which augmented all parameters (85.4+/-10.1, 50.3+/-12.7, 66.5+/-12.5). Also the higher concentrations of LCT/MCT-diluted etomidate and diazepam increased the percentage of RB positive PMNs compared to the alternative compositions. The percentage of phagocytosing PMNs was less reduced with 20 microg ml(-1) LCT/MCT- diazepam (85.2+/-6.9) than with the same concentration of benzyl-alcohol diluted diazepam (68.8+/-12.2) compared to the control. CONCLUSION: The in vitro effects of diazepam, etomidate, and propofol are dependent on the solvent applied. The tested LCT/MCT preparations reduce the inhibitory effects on the bacterial killing capacity of PMNs found after incubation with propyleneglycol, benzyl-alcohol, or LCT preparations, respectively.
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Authors | J Heine, K Jaeger, N Weingaertner, D Scheinichen, G Marx, S Piepenbrock |
Journal | Acta anaesthesiologica Scandinavica
(Acta Anaesthesiol Scand)
Vol. 45
Issue 2
Pg. 213-20
(Feb 2001)
ISSN: 0001-5172 [Print] England |
PMID | 11167168
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Anesthetics, Intravenous
- Triglycerides
- Diazepam
- Propofol
- Etomidate
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Topics |
- Anesthetics, Intravenous
(pharmacology)
- Diazepam
(pharmacology)
- Erythrocytes
(drug effects)
- Etomidate
(pharmacology)
- Flow Cytometry
- Humans
- In Vitro Techniques
- Neutrophils
(drug effects)
- Phagocytosis
(drug effects)
- Propofol
(pharmacology)
- Respiratory Burst
(drug effects)
- Triglycerides
(pharmacology)
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