SRC transcriptional activation in a subset of human colon cancer cell lines.

Abstract

Human SRC encodes the non-receptor tyrosine kinase pp60(c-Src), which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activity. Nuclear run-on analysis and c-Src mRNA half-life determination demonstrated increased mRNA levels were due to increased transcription of the SRC gene. We also observed decreased c-Src mRNA stability in cell lines that displayed SRC transcriptional activation. Our findings provide the first evidence that SRC transcriptional activation is an important determinant of c-Src expression and activity in HCCLs.

• Authors: S Dehm, M A Senger, K Bonham
• Journal: FEBS letters (FEBS Lett) Vol. 487 Issue 3 Pg. 367-71 (Jan 05 2001) ISSN: 0014-5793 [Print] England
• PMID: 11163360 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• RNA, Neoplasm
• Proto-Oncogene Proteins pp60(c-src)

Topics

• Colonic Neoplasms (enzymology, genetics)
• Genes, src
• HT29 Cells
• Humans
• Proto-Oncogene Proteins pp60(c-src) (genetics)
• RNA, Messenger (genetics, metabolism)
• RNA, Neoplasm (genetics, metabolism)
• Transcriptional Activation
• Tumor Cells, Cultured